EEPOKT OF THE BUREAU OF ANIMAL INDUSTRY. 



April 23 agar culture. 

 May 12 b. i. p. (6) 



119 



ii 



May 22 rabbit 



inoculated 

 May 29 died 



Mayirb. i. p. (bj 



May22b. 



I 



It was found that cultures inoculated from b failed to develop at 

 the assigned temperature, so that this experiment was not continued 

 any farther. It deserves to be mentioned, however, that a rabbit 

 inoculated from the original culture which had been kept at 45 C. 

 for ten days died seven days after inoculation with enlarged spleen 

 and coagulation necrosis in liver. 



A temperature of 43--44 C. was next chosen, and the experiment 

 conducted in the same manner, as the appended table will show : 



May 16 



culture. 



May 16 agar culture 



agar 



May 29 b. i. p. (b) (43*^4 

 July 14 placed at SO-38 C. 



July 25 rabbit 

 July 30 dead 



C.) 



August 4 rabbit August 4 b. i. p. 

 (remains well) (sterile) 



