122 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



In the lungs was found broncho-pneumonia, localized chiefly ia 

 the ventral and cephalic lobes. In the larger bronchi lung worm& 

 were present. Superficial inguinal as well as the mesenteric glands 

 were swollen and slightly congested. The spleen was but mod- 

 erately enlarged. In the upper portion of colon were two small 

 superficial ulcers. The mucosa was otherwise intact as far as the rec- 

 tum. In the latter situation it was covered with a peculiar grayish 

 deposit, soft, almost like lard, without tenacity. It was arranged 

 as isolated round patches, from one-eighth to one-fourth inch across 

 and about one-sixteenth inch thick ; the mucous membrane for a dis- 

 tance of 18 inches from the anus being thickly covered with them. 

 They are easily removed and leave a slight depression in the mucosa 

 without apparently any loss of substance. This exudate is made up 

 of degenerated cells, probably epithelial in origin, but they are too 

 far broken down to allow any closer examination. Immense num- 

 bers of bacteria in this deposit of two kinds chiefly; a rather large 

 oval bacillus, with periphery more deeply stained and a very slender 

 rod. 



From this exudate a rabbit was inoculated (September 11) by stir- 

 ring it up in sterile water thoroughly and injecting some of the 

 liquid under the skin of one thigh. The rabbit was dead on the sixth 

 day. There was an extensive sanguinolent staining of the subcutis 

 of the entire abdomen, and of a part of the thorax and thighs. 

 Groups of petecchise on the caecum. Some of the coils of intestine 

 lightly glued to abdominal wall. Spleen small. The peritoneal sur- 

 face is covered with minute cocci ; the same are present in the blood. 

 Agar cultures from the peritoneal surface and heart's blood con- 

 tained the same organisms. Although polar staining was not mani- 

 fest, there was no doubt that the microbe was the non-motile swine 

 plague germ. 



At the same time a rabbit was inoculated in the same way with 

 lung tissue. This animal died in two days. The subcutis of inocu- 

 lated thigh slightly blood-stained. The superficial muscular fiber of 

 a grayish homogeneous appearance. The fascia and muscles of the 

 contiguous portion of the abdominal wall opaque, infiltrated. On the 

 coils of intestine a few small masses of a whitish exudate. Spleen 

 slightly enlarged; liver very much engorged. No bacteria could be 

 distinctly seen on cover-glass preparations from liver, spleen, and 

 blood. JBut tubes inoculated from blood and spleen contained on the 

 following day only swine plague germs. Roll cultures made from, 

 the lung tissue of the pig were liquefied completely in a few days. 



The examination of the spleen gave quite different results. Al- 

 though the microscope did not reveal any bacteria on one coyer-glass 

 preparation from the spleen, three liquid cultures into which a bit 

 of spleen had been placed contained on the following day actively 

 moving hog cholera bacilli. On the surface of an agar culture a con- 

 siderable number of colonies of the same organism made their appear- 

 ance. A roll culture made from one of the liquid cultures contained 

 about 300 colonies, non-liquefying, all alike, and resembling in growth 

 hog cholera bacilli. 



It was very important to determine the pathogenic character of 

 this bacillus in order to confirm the diagnosis based upon its appear- 

 ance under the microscope and in cultures. 



September 13. Two mice were inoculated subcutanepusly, each with 

 several drops from one of the liquid cultures from which the roll cult- 

 ure had been made. One mouse was dead September 19, with spleen 



