KEPORT OF THE BUREAU OF ANIMAL INDUSTRY. 123 



enlarged, and in it numerous hog cholera bacilli; the second mouse 

 remained apparently well and was killed fifteen days later. Its spleen 

 was enormously enlarged, and it would no doubt have succumbed in 

 a few days. But the t olio wing intravenous injection from an agar 

 culture which was inoculated from the original spleen agar culture 

 is sufficient to set aside all doubt as to the nature of the spleen ba- 

 cilli: 



November 12, 1888. Pig No. 90, black and white, about five months old. The 

 right crural vein was exposed by raising a triangular flap of skin over it after 

 thoroughly disinfecting the latter with a 1:500 solution of mercuric chloride. Five 

 cubic centimeters of a beef-infusion peptone culture inoculated from an agar cult- 

 ure about a week old was injected into the exposed vein with a hypodermic syringe 

 thoroughly disinfected with 5 per cent, carbolic acid. The liquid culture was two 

 days old when used. Two hours after the inoculation the temperature had risen 

 from 103f F. to 107 F. November 13, there was no swelling, but a slight serous 

 oozing at the place of inoculation. The appetite was good. November 14, at 3 p. 

 in., the temperature was 107|. The animal was disinclined to move, although it 

 came to eat in the morning and evening. November 15, it lay on its side, quietly, 

 with occasional kicking. Found dead at 4 p. m. Autopsy held immediately. 



General blush on skin of ventral aspect, snout, and lips. No swelling at the point 

 of inoculation; slight blood extravasation. Spleen enormously enlarged, 14 inches 

 long, two-thirds inch wide, and one-half to 1 inch thick, gorged with dark blood, 

 and friable. Superficial inguinals enlarged, cedematous; on section diffuse pale 

 red spots; cortex congested. Bronchial and renal glands enlarged, partly hemor- 

 rhagic; gastric glands hemorrhagic throughout substance. The blood is thick, dark 

 colored, coagulation slight, even after several hours' exposure to the air. Several 

 petecchise on epicardium of right auricle. Right side of heart distended with blood. 

 In it a small white clot. Left heart contracted, empty. Lungs normal, excepting 

 one- third of left ventral lobe, which is collapsed, itidneys enlarged, deeply con- 

 gested throughout. The surface is thickly dotted with minute deep red points. 

 The papilla3 so deeply reddened that any extravasations would not be recognizable. 

 A- few petecchias in pelvis. Bladder contains about 30 grams of urine tinged with 

 blood. The whole mucosa of stomach is deeply congested. In fundus it is hemor- 

 rhagic, with numerous patches of necrosed epithelium one-quarter to one-half inch 

 across. The upper 8 inches of duodenum in the same condition as the fundus of 

 stomach. Numerous red points scattered over mucosa of entire small intestine. In 

 lower ileum a few hemorrhagic points. The mucosa of caecum and upper colon very 

 slightly congested, but the remaining two-thirds intensely so. Hemorrhage here 

 and there sufficient to stain the feces with blood which were otherwise normal. 

 The mesenteric and meso-colic glands all deeply congested throughout their sub- 

 stance. 



Cover-glass preparations from spleen pulp showed a large number of hog cholera 

 bacteria. Cultures from the same revealed the presence of the same organisms 

 only. 



September 19. Pig No. 2, alive in the morning, dead at noon. Ex- 

 amined soon after death. Slight reddening of skin on throat, inside 

 of limbs and pubic region. Spleen very large, engorged, softened. 

 Lymphatic glands generally enlarged but pale, excepting the retro- 

 peritoneal glands, the cortex of which is deeply congested. Liver 

 imparts a harsh sensation to the knife. Kidneys normal. Ecliino- 

 rhynchi in jejunum attached to ulcerous depressions in the mucosa. 

 Lowest 12 inches of ileum contain several deep ulcers one-fourth 

 to three-fourths inch across. The mucosa of the ileo-ceecal valve is 

 thickened, ulcerated superficially, and indurated beneath. There are 

 several large ulcers near the valve and in the upper colon, from one- 

 half to 1-J inches across. The corresponding serosa inflamed and at- 

 tached to neighboring organs. 



All but the caudal and dorsal half of the principal- lobe of each 

 lung solidified. The diseased principal lobe has a bluish-red, the 

 other lobes a pale grayish-red color. In the former the infiltration 

 is in the form of minute whitish plugs in a red ground. The latter 



