124 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



on section has a glistening, half -gelatinous aspect. The cut bron- 

 chioles exude a milky, purulent fluid. 



Bacteriological examination of lungs showed in stained cover-glass 

 preparations a large number of pus cells and polar-stained bacteria, 

 located chiefly around the nuclei of the larger cells and probably em- 

 bedded in the now disintegrated cell protoplasm. A few large bac- 

 teria present. Of two roll cultures from this grayish lung tissue one 

 was completely liquefied, the other partially so. A rabbit inoculated 

 from the same tissue (grayish) died in four days. 



The fascia of the inoculated thigh was much thickened, whitish, the subjacent 

 muscular tissue discolored, pale. Intense peritonitis manifested by a grayish exu- 

 date covering large intestine and liver ; the serosa of the former was extensively 

 ecchymosed and glued to the ventral abdominal wall. The exudate consisted Chiefly 

 of leucocytes and immense numbers of polar-stained swine plague bacteria. These 

 were very scarce in blood. An agar culture from the latter contained only swine 

 plague bacteria. A rabbit inoculated at the same time with the red hepatized tissue 

 was ill subsequently, but recovered. It was killed on the sixteenth day. An ex- 

 tensive subcutaneous abscess was found over the abdomen, with necrosis of the skin, 

 which was converted into a dried mass hard as a board. Spleen very slightly en- 

 larged. 



From a bit of spleen pulp from the pig, which was engorged, dark, 

 and enlarged, a roll culture was made. About one hundred colonies 

 of hog cholera bacilli developed. Two beef -infusion peptone tubes 

 into which spleen bits were placed contained on the following day 

 hog cholera and butyric bacilli. To test the virulence of the hog 

 cholera bacilli, two or three drops of one liquid culture were injected 

 beneath the skin of two mice September 28. One died prematurely 

 on the following day with enlarged spleen, owing to the extensive 

 invasion of liver and kidneys with coccidia, and consequent degener- 

 ation of these organs. The second mouse died in three days with a 

 large number of tcenia in the dilated duodenum. Both died no doubt 

 from the inoculation, but their death was hastened by the presence of 

 the parasites, making the demonstration unsatisfactory. 



Another pig (No. 3) which had been found dead in the morning 

 and undergone considerable decomposition was examined superfi- 

 cially at the same time. The spleen was very much enlarged ; lungs 

 normal. The large intestine contained extensive ulcers with local- 

 ize^, peritonitis and adhesion to surrounding structures. No bac- 

 teriological examination was made. 



A third pig from the same herd (No. 19) was taken, while sick, to 

 the Experiment Station of the Bureau in order that fresh pigs might 

 be infected and our study of the disease continued. It was found 

 dead September 24, four days after its arrival. This animal also 

 manifested both diseases, more prominently so than those already 

 described. Both germs were found in its body, the hog cholera 

 bacillus in the spleen, the swine plague coccus in the lungs, as the 

 following notes show: 



Autopsy several hours after death. No skin discoloration. Spleen very large, 

 due to blood engorgement. Lymphatic glands of abdomen in general very much 

 enlarged, and slightly congested, excepting those of mesentery. Kidneys normal. 

 Liver slightly cirrhosed, somewhat gritty on section. Mucosa of empty stomach cov- 

 ered with yellow bile-stained mucus. Small intestine not diseased. In the caecum 

 and upper 12 inches of colon, about a dozen ulcers, one-half to 1| inches across, with 

 a dirty blackish floor. The ulceration has produced inflammation of the serosa and 

 thickening of the wall. 



Lungs extensively diseased. Of the right, the caudal half of the cephalic, the 

 entire ventral, and the cephalic (anterior) third of the principal lobe hepatized. Of 

 the left lung the same regions, including the azygos lobe, are in the same condition. 



