EEPOET OF THE BUKEAU OF AIOMAL INDUSTRY. 125 



Over these diseased lobes the pleura is covered with a translucent, very thin exudate, 

 either in the form of dots or a mesh-work. Only a little of the adjacent normal lung 

 tissue has the pleura roughened. The exudate is made up of leucocytes and a large 

 number of slender bacilli. The diseased lung tissue, from the surface, is red> mot- 

 tled with minute grayish dots; this mottling is faint in some regions, in others the 

 dots seem to coalesce into grayish patches. The cut surface is grayish red. When 

 compressed, whitish, semi-solid plugs are forced out of the air tubes. In some places 

 these are replaced by a milky, flaky fluid. They consist of leucocytes and epithelium 

 with -very few bacteria. No polar-stained bacteria observed. The bronchial and 

 tracheal glands very large, tough, pale pink on section. The trachea and bronchi 

 coated with bright red foam. In the ends of the latter some lung worms. 



Pericarditis probably an extension of the pleuritis. Both sides of heart and large 

 vessels contain white thrombi. 



A roll culture in gelatine from lung tissue developed a few fungi only.* A rabbit 

 inoculated with lung tissue died in four days. The subcutis of the inoculated thigh 

 much thickened with infiltrated cells (suppuration); over the abdomen also thick- 

 ened and blood-stained. The caecum studded with hemorrhagic points and covered 

 with a gelatinous exudate, which is also found on liver. A cover-glass touched to 

 this exudate contains immense numbers of polar-stained bacteria; very few in blood 

 and spleen. Agar cultures from these develop in moderate number colonies of 

 swine plague bacteria. 



From bits of spleen pulp of the pig an agar culture, a gelatine roll culture, and a 

 liquid culture were made. The agar and the liquid culture contained the motile 

 hog cholera bacilli; the latter also spore-bearing butyric bacilli. The roll contained 

 but two colonies. Of two mice inoculated from the liquid culture one died on the 

 folio whig day; the other in five days, with enlarged spleen, containing hog cholera 

 bacilli in considerable numbers, which were also obtained pure in an agar culture. 

 This mouse had its liver and kidneys thoroughly infested with coccidia. 



At the same time a pig from another herd near Baltimore was ex- 

 amined. The owner had purchased ten young pigs in May. They 

 began to cough and gradually emaciate about one month ago; since 

 then five had died. One of the survivors, emaciated, very weak, 

 with arched back, " tucked-up" abdomen, and dull, sunken eyes, was 

 killed for examination. 



The spleen was not enlarged. The lymphatics in general were enlarged, pale, and 

 firm. Lungs normal, with exception of two or three collapsed areas from one-half 

 to three-fourths of an inch across. Liver and kidneys normal. Catarrhal condition 

 of stomach. In the jejunum a number of ulcers, to two of which echinorhynchi are 

 attached. These worms are very likely the cause of the ulcers. In the cascum and 

 upper colon, especially on and around the valve, were about twenty ulcers, one-half 

 inch across. The slough fell out of most of them while the specimens were being 

 carried from Baltimore to Washington. The ulcers deprived of the slough are 

 nearly circular; the base formed by the muscular wall; the sides vertical, as if the 

 mucosa had been punched out, the border being slightly thickened. The slough 

 itself was yellowish, crumbling. A rabbit inoculated with some of it stirred in 

 sterile water dies in twenty-four hours with slight peritonitis, internal organs gener- 

 ally congested and containing numerous oval swine plague bacteria. A liquid cult- 

 ure from the heart contained only swine plague bacteria, while a gelatine tube cult- 

 ure from the spleen failed to develop. 



From the spleen two tubes of nutrient liquid contain a motile bacillus not dis- 

 tinguishable from hog cholera bacilli. A gelatine roll culture contains about six 

 colonies of the same organism. 



To test the pathogenic power of this bacillus two mice were inoculated from the 

 liquid culture. One died on the following day, with numerous hog cholera bacilli 

 in spleen and liver. Premature death, due to extensive degeneration of these two 

 organs, which was caused by coccidia. The second mouse died on the seventh day, 

 with enlarged spleen and extensive coagulation-necrosis in liver. Both organs con- 

 tain numerous hog cholera bacilli. An agar culture from the spleen confirmed the 

 microscopic diagnosis. 



We have thus four cases in which both hog cholera and swine 

 plague bacteria are present according to the unequivocal results of 

 bacteriological examination. The same was observed in the out- ( 



* This swine plague germ did not, as a rule, grow in gelatine at the ordinary tem- 

 perature of the room. 



