TCEPORT OF THE BUKEAU OF ANIMAL INDUSTRY. 133 



some time with only a small portion of the lung tissue capable of 

 performing its function: 



No. 463 had been exposed to hog cholera at the beginning of 1888, but did not 

 take the disease. It was used subsequently for experiments on purgatives, and 

 dosed with calomel several times. October 1 it was placed in the infected hog 

 cholera and swine plague pen. It had been thin and unthrifty for several months 

 previous, and failed to grow in weight. After staying in this pen for more than 

 three months, apparently resisting infection, it suddenly died January 14. The ab- 

 dominal organs were in general healthy, excepting the liver, which was cirrhosed. 

 When cut a gritty sensation was imparted to the hand. The stomach was also ca- 

 tarrhal. There were no ulcers in the intestinal tract. 



The lungs were extensively diseased. All but about one-fourth of the right lung 

 and one-sixth of "the left lung solid. These regions were situated on the dorsal as- 

 pect of the principal lobe, and were very hyperasmic. The remainder of the lung 

 tissue (with exceptions to be given) is converted into a yellowish- white, homogene- 

 ous mass, having the consistency of rubber to the touch, and cutting like moder- 

 ately hard cheese. Scraping brings away scarcely any of the tissue. The inter- 

 lobular tissue and small bronchioles show very faintly or are effaced. In several 

 places the hepatized tissue is breaking down into a pasty, grayish mass. In the left 

 principal lobe a sequestrum, nearly 2 inches in diameter, of a putty-like consistency. 

 The right ventral lobe is also well-nigh converted into the same pasty material, and 

 the center of the azygos lobe is softening. 



The various lobes are bound together by tough fibrous tissue. The pleura is every- 

 where thickened by the formation of bands and patches of fibrous tissue. 



In order to test the pathogenic effect of this germ, the following 

 inoculations were made in addition to those upon rabbits already de- 

 scribed: 



Two fowls which were inoculated into the pectoral with 1 cubic centimeter each 

 of a liquid culture showed no signs of disease. Two pigeons inoculated with | cubic 

 centimeter each likewise resisted successfully. 



Two mice received about ^ cubic centimeter of a liquid culture under the skin 

 near the root of the tail. One died on the second, the other on the third day after 

 inoculation. In the spleen and heart's blood of both were large numbers of polar- 

 stained swine plague bacteria. In one of them a gelatinous exudate on the pleura 

 consisted of immense numbers of these germs, together with many leucocytes, whose 

 protoplasm was gorged with them. The lungs were in part hepatized. (In both 

 mice the duodenum was greatly distended by masses of tape-worms; in one the liver 

 and kidneys were far advanced in fatty degeneration. These pathological conditions 

 no doubt hastened the fatal result.) 



Effect on pigs. Our past experience with this germ is that it is 

 difficult to predict results of inoculation into pigs unless large quan- 

 tities are injected. This is particularly true when the germs are 

 obtained from an outbreak of a mild character, as was the case with 

 the one under consideration. The following inoculations without 

 any result confirm this past experience:* 



October 8. From an agar culture obtained from a rabbit inoculated with lung 

 tissue (pig No. 9), the condensation water containing an abundant growth of swine 

 plague bacteria was mixed with 10 cubic centimeters of sterile bouillon. A bouil- 

 lon peptone culture from the same source was added to this, making 20 cubic 

 centimeters in all. One pig (No. 36) was inoculated subcutaneously with 10 cubic 

 centimeters of the culture liquid, one-half into each thigh. No. 37 was inoculated 

 into the right lung through the chest wall, receiving 3 cubic centimeters. No. 38 

 was inoculated in the same place, 2 cubic centimeters being injected. 



No. 38 manifested no ill effects, being watched several months. No. 37 likewise 

 remained well. No. 38 began to decline about a month after the inoculation, and 

 three months after appeared stunted and unthrifty although it ate very well. 

 Owing to its poor condition it was killed January 23. There were no lesions of ab- 

 dominal or thoracic organs, excepting a few membranous expansions of connective 

 tissue fastening the ventral border of a part of the right lung to the chest wall. 

 Closer examination revealed an old pleuritis, indicated by small shreds of fibrous 



* Compare with these similar inoculations made with swine plague germs from 

 a severe Iowa outbreak, reported farther on. 



