138 EEPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



On December 1, two mice and a rabbit were inoculated from an ulcer of the same 

 pig kept in a sterile test tube in the refrigerator. None of these animals showed 

 signs of illness thereafter. 



No. 5, a small pig, said to have died forty-eight hours ago. On examination no 

 lesions were found in lungs or intestines. Two tubes of agar into which bits of the 

 small, pale spleen were put remained sterile. 



No. 6, of the same size as the two preceding, said to have died about forty-eight 

 hours ago. The ulceration in the large intestines was* as extensive as in No. 4. Of 

 the lungs one ventral lobe was hepatized. From the spleen, which was considera- 

 bly enlarged and congested but rather firm, two agar cultures were made. These 

 remained sterile, although cover-glass preparations had shown the presence of strep- 

 tococci. Six days later a rabbit inoculated from an ulcer, meanwhile kept in the 

 cold, remained well. 



The results of inoculations and cultures from these three cases were thus entirely 

 negative. 



Farm C. Tlie disease appeared about November 1 in a herd of 14 

 shoats and 2 old hogs. The shoats, weighing from 125 to 175 pounds 

 each, were all dead before November 20. They had been kept in 

 pens with a small yard to run in, and were being fed on all the new 

 corn and water they could consume, together with skimmed milk 

 and slops from the house. From this herd the following animals 

 were examined : 



November 14, No. 7, black shoat, weighing about 125 pounds, just dead, the vis- 

 cera still quite warm. The animal is in very good condition, although the owner 

 claimed that it had lost at least 50 pounds during its illness. The lungs are perfect 

 excepting the tip of one ventral lobe, where a portion as large as a small marble is 

 solid . Superficial inguinal glands very large and w r ith cortex hemorrhagic . Glands 

 of lesser omentum in the same condition. The lower coils of the ileum show under 

 the serosa punctif orni extravasations ; the walls are thickened and the mucosa itself 

 is concealed by patches of soft yellowish-white exudate about one-tenth inch thick. 

 It is easily peeled off and exposes a deeply reddened membrane. The large intes- 

 tine, beginning with caecum, is extensively ulcerated, the ulcers being in general 

 shallow and extended. From the spleen, which was very large and, gorged with 

 blood, two bits of tissue were placed in agar tubes. These remained sterile. No 

 bacteria could be detected in spleen tissue with the microscope. 



From one of the ulcers a rabbit was inoculated November 20 by placing a small 

 bit of the ulcerated tissue under the skin and drawing the incision together with a 

 single stitch. The animal died in six days. The subcutis of the inoculated thigh 

 was considerably infiltrated, on the abdomen only slightly so. On the opposite 

 thigh near the pubis a small patch of hemorrhagic points on the muscular tissue. 

 No peritonitis. Spleen congested, but barely augmented in size. In spleen and 

 blood no bacteria could be detected; the leucocytes there contained the stained par- 

 ticles, as before described. Two agar and two bouillon peptone tubes were inocu- 

 lated from the blood and spleen pulp. All four tubes remained sterile. 



No. 8, animal about the size of No. 7, died last night. Superficial inguinal 

 glands with cortex reddened, and occasional hemorrhagic points in medulla, which 

 has a decided greenish-yellow tinge. 



All but a narrow portion along the dorsal region of each lung solid, twice as large 

 as in the collapsed condition. No pleuritis. The entire hepatized tissue contains 

 whitish, cheesy masses, from 1 to 4 millimeters (one twenty-fifth to four twenty- 

 fifths of an inch) across, which are consistent, so that the whole lung cuts like liver 

 tissue. The masses are so numerous as to leave but little reddened lung tissue be- 

 tween them; odor moderately putrefactive. Liver has a peculiar mahogany color, 

 indicating stasis of the bile in the bile capillaries and ducts. The acini are plainly 

 outlined, bloodless. Spleen exceedingly large, due to engorgement with blood. In 

 the caecum a large ulcer near the valve, about H inches across, besides six or seven 

 smaller ones one-fourth to one-half inch across, the yellowish slough slightly pro- 

 jecting. 



From the lung a rabbit was inoculated November 21. A bit of lung tissue was 

 teased and broken up in sterile bouillon and injected subcutaneously into the thigh. 

 The rabbit was dead on November 24. The injection had caused considerable pasty 

 thickening of the subcutis on the thigh. The spleen was enlarged and dark colored. 

 Other lesions absent. Cover-glass preparations fail to show bacteria in spleen, liver, 

 and heart's blood. From the spleen and the blood each an agar and a bouillon pep- 

 tone tube were inoculated. The two agar and one bouillon tube contained, on the 



