REPORT OF THE BUREAU OP ANIMAL INDUSTRY. 139 



following day, growths which proved to be made up only of swine plague bacteria. 

 The other bouillon tube contained also a short non-motile bacillus. 



Another rabbit had been inoculated at the same time by placing a small bit from 

 one of the ulcers of the caecum under the skin on the inner surface of the thigh. 

 This animal was found dead November 27. There was in this case but slight sup- 

 purative infiltration of the subcutis at the point of inoculation and a few petecchiae on 

 abdominal muscles. Spleen small, dark; liver congested and in an advanced state of 

 fatty degeneration. Cysticerci in omentum. In the spleen, liver, and blood an im- 

 mense number of swine plague bacteria, showing very clearly the polar stain. Cult- 

 ures in agar and in bouillon peptone from the spleen and heart's blood contained 

 on the following day only swine plague bacteria. A tube of gelatine inoculated at 

 the time failed to grow. Swine plague bacteria were thus obtained from both in- 

 testine and lungs of No. 8. 



No. 9, in size and weight like the two preceding. So sick that it was thought 

 best to kill it. In this animal there was no ulceration of the large intestines, but 

 in the ventral portion of the left lung the broncho-pneumonia, so advanced in No. 

 8, was already established. The spleen was moderately engorged with blood. Two 

 agar cultures" from spleen bits remain sterile. From a bit of lung tissue a rabbit was 

 inoculated into the thigh subcutaneously on November 21. It was found dead No- 

 vember 25. The local lesion involves the subcutaneous connective tissue of thigh 

 and abdomen. The muscular tissue underneath is grayish, discolored. The sub- 

 cutis overlying it is thickened and softened, partly gelatinous, and infiltrated with 

 blood in the groin. The caecum directly underlying the subcutaneous inflammation 

 of the abdomen is dotted with hemorrhages, and about 3 inches of the blind end has 

 its walls very much thickened, softened, the serous aspect discolored with blood ex- 

 travasations, and the mucosa grayish, softened, the contents of a semi-gelatinous, 

 colorless aspect. Slight exudate on the ventral surface of the liver. The subcuta- 

 neous and peritoneal ^xudates contain an immense number of cocci ; in the blood 

 they are few in number. None show any distinct polar stain. Agar cultures from 

 the peritoneal exudate and from heart's blood contain, on the following day, a 

 growth of swine plague bacteria only. 



No. 10, small shoat, died only a few hours ago. All of left lung but a small strip 

 of dorsal region hepatized. The principal lobe is in a condition of dark red hepati- 

 zation interspersed with areas of a paler color. Over the ventral lobe, on its lateral 

 aspect, an exudate nearly one-eighth inch thick, lamellar, easily scraped away as a 

 yellowish -white pultaceous mass. The lung tissue beneath it in a state of advanced 

 cellular infiltration (broncho-pneumonia). The ventral lobe of the right lung is 

 likewise involved in broncho-pneumonia. In the caecum near the valve a slightly 

 depressed, ragged, gnawed ulcer about 1 inch across. The mucosa of the entire valve 

 is also ulcerated. No lesions lower down. From the slightly congested but not 

 enlarged spleen two agar cultures were made. These remained sterile. 

 . On November 21 a rabbit was inoculated subcutaneously with one- fourth cubic 

 centimeter of an emulsion of the lung tissue in sterile beef infusion. This rabbit re- 

 mained alive and apparently well. December 17, nearly a month later, it was killed 

 with chloroform. A large ulcer had formed on abdomen, exposing the thickened 

 subcutis firmly attached to subjacent muscular tissue. No peritonitis. Spleen small 

 and pale, liverdark. Stomach well filled with food. In the walls of th* caecum near 

 the blind end about a dozen whitish patches, the largest about three-sixteenths of an. 

 inch across, the smallest barely visible. These patches correspond to soft white 

 masses, probably lymph follicles undergoing suppuration. In the left lung a small 

 area of hepatized tissue. The process in this case had been localized in the subcutis, 

 and the condition in the caecum may have been due to embolism. 



On December 1 a second rabbit wa? inoculated in the same way from the same 

 material, kept meanwhile in the refrigerator. The result was entirely negative. 



The virulence of pure cultures of the swine plague bacteria obtained from pigs 

 Nos. 8 and 9 by inoculation into rabbits was tested about a month later on rabbits 

 by suspending- a loop full of the gelatinous growth from the condensation water of 

 agar cultures in about 1 cubic centimeter of sterile water, and injecting one-eighth of 

 this amount subcutaneously. Rabbit inoculated from culture from pig No. 8, Jan- 

 uary 2, dead next morning in less than twenty hours. Slight infiltration of the sub- 

 cutis of inoculated thigh with ecchymoses and discoloration of the subjacent 

 muscle. Lymph gland of groin hemorrhagic. In the internal organs no changes, 

 no peritonitis. Spleen dark, slightly enlarged. In spleen and liver an immense 

 number of polar-stained swine plague bacteria. 



Rabbit inoculated from culture from pig No. 9, January 8, died within forty-eight 

 hours. The lesion at the place of inoculation somewhat more pronounced and ex- 

 tending to abdomen. In the latter cavity the csecum and portion of colon densely 

 sprinkled with subserous punctiform extravasations, portions of rectum and duo- 



