144 EEPOET OF THE BUEEAU OF A1STIMAL INDUSTEY. 



This experiment shows the different effect of inoculation, under the 

 skin and into the thorax, of the same quantity of culture-liquid. 

 While there was no effect from the former, the latter caused an 

 acute septicaemia fatal in less than twenty-four hours. 



A second experiment was tried in order to obtain, if possible, the 

 lung disease as found in spontaneous cases. 



December 6, two pigs (Nos. 43, 47) were inoculated into the thorax 

 as already described, No. 43 receiving !- cubic centimeters of a 

 bouillon peptone culture originally derived from Iowa pig No. 1, and 

 No. 47, 3 cubic centimeters of the same culture liquid. 



No. 47, inoculated at 8 a. m. Temperature on the following day, 4.30 p. m. , 105 F. 

 Ate nothing during the day. Respirations labored, abdominal. December 9, tem- 

 perature, 104^ F. ; scarcely able to stand ; respirations accompanied by a groan. 

 December 11, temperature, 102f; very weak and failing until killed December 11. 

 At the autopsy the spleen was found small, pale. Abdominal organs and lymph 

 glands in general normal. 



The right pleural sac contains a considerable quantity of blood-stained serum. The 

 pleura of the lungs, the diaphragm, and the chest wall of the side highly inflamed, 

 thickened, and covered by a thick, loose, and spongy exudate, easily scraped away. 

 The ventral lobe of the right lung is solid, the hepatization dark red ventrally , with 

 five or six lobules necrosed and of a pale-yellowish color. Dorsally in the same lobe 

 the necrosis is more extensive and very likely represents the region where the needle 

 entered the lung tissue. On epicardium a thick, soft deposit, the pericardium much 

 thickened. The left lung normal, but adherent in several places. Of six cultures 

 on agar made from the pleural and the epicardial exudate all but one are pure 

 cultures of a germ identical with the injected swine plague bacteria. Of three 

 cultures from the spleen one remained sterile; the remaining two contain only swine 

 plague bacteria. 



If this animal had been permitted to live longer the lesions would 

 without doubt have been more extensive in the lung tissue itself. As 

 it was, they are sufficiently severe and characteristic to prove the 

 pathogenic power of the bacteria injected. 



No. 43, which received but 1-J- cubic centimeters (about one-third dram) of the 

 same culture, showed signs of disease immediately by remaining quiet, refusing 

 food, and breathing laboriously. Five days later, temperature still above normal 

 (105f). From this time on it became somewhat better; its appetite returned in two 

 weeks. It did not fully recover, however. One month after the inoculation it was 

 generally unthrifty with staring coat and enlarged abdomen. It was killed January 

 23. The abdominal organs were normal. In the thorax both lungs were found 

 everywhere adherent to the chest wall and diaphragm by a continuous mass of 

 fibrous tissue not yet very firm. The lungs themselves were not diseased. The peri- 

 cardium, however, was very much thickened, and when slit open a mass of white 

 cheesy pus was found under it, entirely encircling the heart near the base. About 12 

 cubic centimeters of this cheesy mass was removed. The inner surface of the thick- 

 ened pericardium was dark, bluish red. Without doubt the needle, instead of pene- 

 trating the lung tissue, had entered the pericardia! sac and deposited a portion of 

 the culture liquid in it, converting it into a veritable abscess cavity. Two agar 

 tubes inoculated from this pus contained a moderate number of colonies which 

 were made up of swine plague bacteria. They had thus remained alive one month 

 and a half. % 



In order to observe the pathogenic effect that might be exerted by 

 this germ when deposited in the abdominal cavity two pigs (Nos. 

 138, 139) received 5 cubic centimeters and J cubic centimeters, re- 

 spectively, in this situation. The material used was a bouillon pep- 

 tone culture twenty-four hours old, made from agar culture originally 

 derived from Iowa pig No. 9. The result was negative so far as any 

 severe effect was anticipated. No. 138, inoculated December 27, re- 

 mained very quiet for several days, and could only be made to get up 

 with difficulty. At the end of the third day the temperature was 

 105 F. At the end of the week it had nearly recovered. 



