EUROTIUM 237 



2. The transversely septate, branched mycelium, from which 

 the conidiophores arise as vertically growing branches, usually 

 from a point immediately behind one of the septa : in this as 

 in other cases of branching of the mycelium, the branch grows 

 out at right angles from the hypha which bears it. 



3. The swollen spherical bead of the conidiophore, with its 

 conidia in radiating rows inserted upon it. Examine carefully 

 the way in which the conidia are produced, noting 



a. The sterigmata, which are peg-like radiating outgrowths 

 from the head of the conidiophore. 



b. The series of conidia, in successive stages of development, 

 which have been successively formed by abstraction from the 

 sterigmata. 



c. The oval form, and spiny surface of the mature conidium. 

 In order to observe the successive stages of development of 



the conidiophore, small portions of the Fungus should be taken 

 from the white patches, where the growth is younger, and be 

 treated as before. In these specimens the following points are 

 to be observed 



1. The conidiophore as a club-shaped thick erect hypha. 



2. The swelling of the head, though it at first remains quite 

 smooth. 



3. Minute papillar outgrowths appear on the surface of the 

 head these are the young sterigmata. 



4. The sterigmata elongate, and become attenuated at the 

 tips. 



5. The successive stages of abstriction of the conidia from the 

 apices of the sterigmata. 



III. In order to trace the germination of the conidia, they 

 should be cultivated under microscopic observation on the 

 slide. For this purpose a moist chamber is to be prepared as 

 directed in Appendix A. It will be necessary to take certain 

 precautions to reduce the probability of access of foreign spores 

 to a minimum, and so insure as nearly as possible a pure 

 culture. Prepare a nutritive solution by boiling French plums 

 in water : this decoction is to be used very dilute, and is to be 

 boiled immediately before starting the culture, so as to kill 

 any foreign spores which may be already present : with the 



