REPORT ON DOUltl 11 



of observation. Inoculated subcutanoously on August 1th 

 1903 with 1-rO c.c. of blood drawn from jugular vein of tho Arab 

 stallion Monarch during the interval between two paroxysms. 

 A diffused swelling followed tbe inoculation at and around the 

 seat of injection, but this had entirely disappeared by the morning 

 of the 8th. After an interval, during which period not even 

 thickening could be felt, a swelling 1 , hard, warm and tender on 

 palpation, became apparent at the seat of inoculation and the tem- 

 perature rose 2'6C. During the two following days the swelling 

 decreased, but thickening remained at the seat of inoculation and 

 persisted until the 6th September 1903. The first symptom of 

 Dourine was the secondary swelling, which appeared at the seat of 

 inoculation on the 13th day. At the same time the trypanosomata 

 were discovered on microscopical examination of the blood drawn 

 from the swelling. The eruption of the first plaque took place on 

 the 33rd day, and the tenth and last on the 50th day. Up to Febru- 

 ary 29th, 1901, the 210th day following inoculation, no further 

 symptom of the disease had become manifest, except that the 

 mucous-membrane of the meatus-urinarius was everted. 



Donkey No. I. A small country-bred donkey mare, aged 5 

 years, was inoculated scbcutancously with 20 c.c. of blood drawn 

 from general circulation of Monarch, during an intermission on 

 the 226th day after the date of the first covering. The primary 

 swelling at the seat of injection was absorbed within eleven days. 

 This was followed by a period of rest lasting eight days, during 

 which no changes were observed. Then a secondary swelling 

 made its appearance, hot and red in colour, which after 82 hours 

 attained a size of 9 x 4-5 x 0'75 inches. The trypanoscma was 

 observed in the fluid drawn from the tumour. The swelling dis- 

 appeared in the course of ten days from the onset, but slight 

 thickening of the skin persisted for a further period of nineteen days. 

 Up to the 81th day no other symptom of Dourine was observed. 



Donkey No. IF. A small entire donkey, country-bred, aged 7 

 years, was subcutaneously inoculated with 15 c.c. of blood drawn 

 from the general circulation of Yadgir during au intermission on 

 the 51st day of one lasting 53 days in all. The primary swelling 

 at the seat of injection was absorbed within four days. 



