ON DOURINB. 18 



Dog No. I. A pariah puppy, aged 4 months, was inoculated 

 on several occasions, but always with negative results. 



(i) Subcutaneous inoculation with - 5 c.c. of blood drawn 

 from plaque No. 2 Shamaher. Trypanosoma present. 



(ii) Subcutaneous inoculation with 10 c,c. of blood from general 

 circulation of Shamher. Few hours after eruption of plaque. 



(iii) Subcutaneous inoculation with 1*5 c.c. of sero-sanguinous 

 fluid from oedematous swelling on the under-surface of the abdomen 

 of Shamsher. 



Dog No. II. A pariah puppy, aged 4 months, was subcuta- 

 ueously inoculated on several occasions, but in each instance with 

 negative results. 



(i) Subcutaneous inoculation with 5 c.c. of blood from the 

 general circulation of Shamsher during intermission between plaques 

 Nos. 2 and 3. 



(ii) Subcutaneous inoculation with 0'45 c.c. of blood from 

 plaque No. 4 Shamsher. Trypanosoma present. 



(iii) Subcutaneous inoculation with I/O c.c. of blood from plaque 

 No. 5 Shamsher. Trypanosoma present. 



(iv) Subcutaneous inoculation with 1*0 c.e. of blood from plaque 

 No. 7 Shamsher. Trypanosoma present, 



Dog No. III. A young pariah dog, was inoculated subcutane- 

 ously with 10 c.c. of blood drawn from the general circulation of 

 Kilngarth. Trypanosoma present in the plaques, which persisted 

 at the time of the collection of blood. The dog was kept under 

 observation for a period of six months, daily microscopical examina- 

 tion of the blood being made, but with negative results. Body 

 weight of the animal increased from 29 to 84 Ibs. during the 

 period of observation. 



Dog No. IV. A young pariah dog, was inoculated in the 

 anterior chamber of the eye with 4 drops of blood drawn from 

 plaque No. 12 (e) Kilngarth. Trypanosoma present. No symp- 

 toms of Dourine developed during the following six months. 

 Blood examined microscopically daily. Body weight of the animal 

 increased during the period of observation from 26 to 34- Ibs. 



Rabbit I. A white rabbit, body weight 953 grammes, was sub- 

 cutaueously inoculated with 4 c.c. of blood k drawn from general 



