14 REPORT ON DOURINE. 



circulation of Kiln garth. Blood examined for four months, no 

 symptoms of Dourine supervened. Body weight increased to 1920 

 grammes. 



Rabbit 7/. A white and black, body "weight 1440 grammes, 

 was inoculated in the anterior chamber of the eye with 0'2 c.c. of 

 blood drawn from plaques No. 12 (c) Kilngarth. Trypanosoma 

 present. No symptoms observed until the 49th evening after 

 inoculation, when temperature registered 39 '0 C. followed by 

 41'3 C. on the 50th morning. Death on the 53rd day. Trypano- 

 eoma present in very small numbers in blood of general circulation. 

 Increase of 90 grammes in body weight during period of observa- 

 tion. 



Gmnea-pig No. I. Body weight 603 grammes, was subcuta- 

 neously inoculated with three drops of blood containing the try- 

 panosoma, drawn from plaque No. 12 (c) Kilngarth. Daily micro- 

 scopical examination of the blood during a period of over 6 months 

 failed to reveal the presence of the trypanosoma in the general 

 circulation. Body weight 700 grammes. 



Section III FACTS BEARING ON THE ETIOLOGY OF DOURINE. 



(I) Period of incubation in Dourine. It is a most difficult 

 matter to accurately determine the period of incubation in spon- 

 taneous cases of this disease, that is the period which elapsed between 

 the first covering or inoculation of the vulva and the appearance of 

 the primary symptoms. In some cases, an external manifestation, 

 such as the sudden enlargement of one or both of the labia- pudendi, 

 is noticeable, but in others no such symptom is exhibited. From 

 our previous investigations with the trypanosoma of Surra, we 

 know that in some animals a distinct tumour may form at the seat 

 of inoculation and may persist until the trypanosoma escapes into 

 the general circulation of the animal, but on the other hand no such 

 local change need take place at the seat of inoculation, and yet the 

 animal becomes the subject of the generalized disease in due course 

 after a period of 4 to 7 days. 



The primary or spontaneous cases of Dourine in stallions have 

 helped us but little in forming an opinion regarding the period of 

 incubation, as notes of course are not kept or information forth" 



