KEPOET ON DOURINB. 29 



day, but between the 7th August and 15th September 190tJ, the 

 93rd to 182nd inclusive, a period of 39 days, a systematic examina- 

 tion of staine.d cover-glass specimens was made daily, in order to 

 determine the number of trypanosomata present. During this 

 period nine paroxysms and nine intermissions occurred. The former 

 occupied 15 and the latter 25 days. The duration of the paroxysms 

 varied from I to 4 and the intermissions from 1 to 6 days. The 

 number of trypanosomata during the paroxysms were found to vary 

 in the small quantity of mucus examined on each occasion from 

 1 to 2,000 in a cover-glass specimen. 



Mare VII. During the first seven weeks no protozoa were 

 discovered in the vaginal mucus. (August 7th to September loth.) 

 But during the following 27 days eight paroxysms and eight 

 intermissions occurred, the former occupying 12 and the latter 

 15 days, the duration of the different periods being 1 to 8 days 

 respectively. The trypanosomata varied during the paroxysms 

 from 2 to 278 in a cover-glass specimen. 



Mare VIII. Observations conducted daily between 7th July 

 and loth September 1903 inclusive demonstrated that during the 

 first 14 days no trypanosomata were present in the vaginal mucus. 

 But during the following 57 days ten paroxysms and teu inter- 

 missions took place, the former occupying 12 days and the latter 

 45 days. The protozoon was discovered at intervals in varying 

 numbers of 1 to 6,48U in a cover-glass specimen up to the end of 

 February 1904, when the observation ceased. The trypanosoma 

 was not observed on microscopical examination in anything but 

 the vaginal mucus ; in the muco-purulent discharge it was searched 

 for on several occasions, but never discovered. As soon, however, 

 as an interval occurred in the secretion of the latter, the trypano- 

 soma was usually found in the unmixed vaginal mucus. From the 

 above table it may be observed that trypanosoma was found in the 

 vaginal mucus, as early as the 17th or 18th day after covering; 

 perhaps it may have been present even before that date. At times 

 clear, glairy mucus dropped from the lower commissure of the 

 vulva, and in this the trypanosoma was found ; on the other hand, 

 great difficulty at times was experienced in obtaining sufficient 

 mucus to place on a cover-glass for microscopical examination. 



