REPORT ON DOURINE. 88 



two, however, do not coincide, for it frequently happens that subcu- 

 taneous plaques appear on the days during which the trypano- 

 somata are absent from the vaginal mucus. Microscopic examin- 

 ation of the mucus elicits the fact that the trypanosomata may bo 

 present in small or in vast numbers, and that they in many instances 

 undergo longitudinal division. In some specimens all the recog- 

 nized forms, mature and developmental, are to be observed, includ- 

 ing the irregular and free chromatin bodies. Further, within a 

 few days all mature parasites may have 'disappeared and only the 

 developmental forms be left. To account for this it would appear 

 probable that the destruction of the mature organisms may be due 

 to the formation of a toxin as in the case of the cutaneous plaques. 



X. What changes take place at the seat of sulcutaneous inoc*' 

 lation, in a susceptible animal, when liourine Hood containing (he 

 developmental forms of the trypanosoma is injected. The primary 

 changes depend somewhat upon the quantity of blood injected, so 

 that the symptoms exhibited depend more or less upon the mecha- 

 nical strain to which the tissues were subjected. For example, let 

 us take Donkey I, inoculated with 20 c.c. of blood. On the day 

 following the operation a tense swelling appeared which began to 

 decrease iu dimensions after 36 hours and was finally absorbed in 

 a period of eleven days. On the 19th day slight thickening 

 appeared at the seat of inoculation which persisted for 48 hours. 

 On the 21st day a swelling hot and red appeared at the seat of 

 injection which gradually increased in size until it attained its 

 maximum dimensions 36 hours later on the evening of the 23rd 

 day. On the morning of the 27th day, it commenced to decrease 

 and was finally absorbed by the 32nd day. Thickening of the 

 skin, however, persisted until the 75th day. Stained specimens of 

 blood or sero-sanguinous fluid were examined daily during the 

 above interval of 75 days. Between the first and tenth days 

 inclusive, the changes at the seat of inoculation were of an inflam- 

 matory nature due to the presence of the injected blood. From the 

 llth to the 18th day, a period of inaction intervened. Daring 

 the above periods, no trypanosomata or developmental forms were 

 discovered. During the next twenty days, 19th to 38th inclusive, the 

 fluid collected from the swelling only exhibited the developmental 



