BEPOI1T ON DOURINB. 83 



ween 8th December 1903 and 9th February 191H, i.e., between 

 219th and 282nd day. 



Although the trypanosoma had been observed in the sero- 

 sanguinous fluid removed from the swelling at the Feat of inoculation 

 on the 5th January 1904, no plaques made their appearance on the 

 cutaneous surface of the body until 24-th Mny, seven d;iys after par- 

 turition, and consequent loss of power of resistance which followed. 



A period of seven clays elapsed between the disappearance 

 of the temporary swelling caused by the injection of the blood 

 subcutaneously in the first instance, and the appearance of the 

 secondary swelling at the seat of inoculation 'due to the develop- 

 ment of the trypanosoma in situ. 



Further, a period of seven days elapsed between the birth of 

 the foal and the rise of temperature and swelling of the right 

 fore-knee. 



The foal has exhibited to date no symptoms of disease, so 

 it will be inoculated with the Dourine trypanosoma in order to 

 ascertain if any protection has been afforded in uterti. 



Present condition: October 18th, 1904. Good. No symptoms Donkey sun 

 . . kl . NO. in. 



visible. 



This experiment demonstrates that, although the trypanosoma 

 of Dourine could not be found microscopically in the blood tf the 

 affected animal at the time of inoculation, nevertheless when a 

 relatively large quantity was injected subcutaneously into a 

 healthy animal (Donkey) not only was the disease contracted by 

 the inoculated animal, but plaques appeared during the early days 

 after inoculation (33rd) and aversion of the mucous-membrane 

 of the meatus-urinarins occurred at a later date. This is the first 

 occasion on \*hich Dourine plaques have been noted in donkeys 

 in this country. 



No symptom other than eversiou of the mtatus-urinarius has Donkey suu 



Vn I v I 



become manifest since the 29th December 1903, but that the * 

 animal is the tubject of Dourine there is no doubt ; up to the pre- 

 sent, however, the " materies morli " has lain dormant. 



Present condition : October 18th, 1904-. Good; no symptoms. Pony*ire 

 On February 29th, 1904, this animal showed no symptoms of" 

 Dourine, and no further ones \vere noted until 9th M;.rch, when 



