APPENDIX. 



Trypanosoma was frequently demonstrated in blood drawn from the plaques of 

 recent date. On reference to the Table it may be observed that the length of 

 the periods intervening between the successive appearance of any two plaques 

 varied considerably ; in the cases of Nos. 3 and 4 and Nos. 17 and 18 plaque s 

 the periods were one and eighty days respectively. No. 1 plaque, which became 

 risible 30th December 1902, was particularly noticeable on account of its bein^ 

 one of the few patches observed which might be called typical and which agreed 

 with the descriptions applied by some observers to 'the Dourine plaques, viz., 

 " As if a metallic disc had been placed under the skin of the animal." In addition 

 to the pathognomonic patches, disseminated urticarial eruptions of a fugitive 

 character made their appearance at varying intervale of two to six weeks ; as a 

 rule implicating a different region of the animal's body during each manifesta- 

 tion. In this case, twenty-four hours to three days embraced the usual limit 

 of their persistence. 



On the 22nd and 25th February 1903, Shamsher was allowed to cover 

 mare I. No sores or abrasions existed on the genital organs. The ejaculated 

 fluid presented the characteristic of water, and when the fluid was submitted to 

 microscopical examination it was found to contain a few epethelial cells, 

 spermatozoa and the trypauosoma being absent therefrom on both occasions. 

 At a later date in March, the animal was tried with a mare, but was unable to 

 corer. The cedematous swelling of the sReath'aud scrotum of the animal, which 

 was marked on its arrival in Bareilly, increased considerably ia dimensions 

 during the early days of March 1903, when it attained its maximum proportions. 

 The tissues then appeared to be on the point of giving way under pressure of. 

 the contained cedematous fluid. It gradually, however, decreased io size and 

 later the innltiated tissues became organized. 



The scrotal wounds continued to suppurate and heal again under treatment 

 at intervals of six weeks to two months, until about the end of August 1903. 

 Since that date all inflammatory symptoms complicating the scrotum and sheath 

 have subsided. 



The external inguinal glands which were enlarged in October 1902 

 gradually decreased in size, and in the following June could not be felt on 

 manipulation. During the last few weeks of 1902 and the first three months 

 of 1904 several of the joints became affected, were the seat of swelling and 

 somewhat painful to the touch, but the symptoms subsided on each occasion 

 after a few days 1 duration. On the 12th December J902 swelling and oedema 

 made its appearance on the under-surface of the abdomen, spreading forward, as 

 in other animals, after increasing and diminishing in amount alternately, and 

 occupying varying periods in the process ; it then disappeared for a term, only 

 to return later, on one or more occasions. On January 31st, 1904, the oedema 

 was still present, having persisted more or less since December 27th, 1903. 



The temperature of this animal has been taken and recorded twice daily 

 during a period of fourteen months. The maximum 40'5C. was noted on the 

 evening of the 2nd March 1903, the morning temperature of the same date 

 being 37'7 C C. This rise may have been due to the fact that on this date the 

 animal was cast for the purpose of careful examination, and the opportunity 

 was embraced for collecting some specimens of blood from the existing plaque 



