XXXIV APPENDIX. 



On the 22nd and 25th February, this animal was covered by Shamsher, an 

 Arab horse, the subject of chronic Dourine, of many months duration. No sores 

 or abrasions existed on the genital organs of the stallion at the time of cover- 

 ing and the ejaculated fluid which presented the characteristics of water only 

 did not contain any trypanosomata or spermatozoa. 



During the period which elapsed between the arrival of the mare at 

 Bareilly, 20th February, and the 10th April 1903, when she was transferred to 

 the Muktesar Laboratory (elevation 7,600 ft.), the rectal temperature only rose 

 to 38-2 C. 



After a period of six weeks had been allowed to elapse from the date of the 

 former covering, and as no symptoms of any kind, the result of the union, 

 manifested themselves, the mare was served on two occasions on the 3rd and 

 8th April by a thorough-bred English horse, Kilngarth,* the subject of Dou- 

 rine for some months past. 



On the 25th April it was observed that the vaginal mucous membrane of 

 mare I was very much congested, and that on the following day the right 

 labium was swollen and oadematous, and that on the 27th the symptoms were 

 still more marked. These symptoms, however, decreased and subsided in the 

 course of the next few days. On the 2nd May, the first plaque made its appear- 

 ance on the right side over the ribs ; it was circular in form, 2'5 inches in 

 diameter and pitted in the centre. It would therefore appear that this mare 

 contracted the disease at the time o or directly after the first union, and that 

 the period of incubation occupied only 30 days. 



Between the 3rd and 7th May inclusive, the plaque gradually decreased 

 in size and prominence ; on the 8th slight thickening of the cuticle over its site 

 occurred, but this was only temporary and the plaque disnppeared on the 

 morning of the 10th. During the interval until the 20th no symptoms were 

 observed, but on that day the vaginal m. m. was observed to be oadematous and 

 the passage patent ; this condition persisted for a period of only 48 hours and 

 then subsided. It, however, recurred in a more aggravate! form on the 31st 

 and continued until the llth June, when it again disappeared. 



On June 19tb, a fresh plaque No. 2 (urticarial) appeared on the left ribs 

 circular in form, 1 inch in diameter, with well raised border. The following 

 morning it had increased in size and measured 4"X S'Xfl'S* and was soft in 

 consistency, but during the following 24 hours decreased so that only a slight 

 thickening of the skin over the former seat of the plaque could be felt. All 

 signs had disappeared on the morning of the 23rd. On this date also the 

 vaginal m. m. again became swollen and cedematous and the submaxillary 

 glands which had up to this time been normal in size, now were found to be 

 enlarged and hard. 



29th June. A. fresh plaque No. 3 (urticarial) oval in form 2'xl'5" 

 appeared on the left side of the neck and persisted for an inordinate period. 



Between June 30th and July 22nd plaque No. 3 remained unaltered, and 

 the oedema of vaginal m. m. varied in amount. The mare during this period 

 was always in a condition of sestrum. On the latter date two fresh plaques 



*Thi animal was received from Major Pease, Principal, Lahore Veterinary College, who has 

 published notes on the initial symptoms observed up to its being sent to Bareilly. 



