MARB IV. 



Scarification of m. m. of R. labium pudendi, and inoculation of fresh blood 

 obtained from a Dourine plaque on English T. B. stallion Kilngarth 

 Tumefaction of both labia on 15th day, and oedema of perineum Ab- 

 tence of the trypanotoma from vaginal mucus First plaque appeared 

 on 70th day following inoculation (presence of trypanosomata demons- 

 trated}, followed at intervals during a period of 105 days by successive 

 crops of plaques numbering 18 in all, which involved the skin of the 

 body and neck Slight enlargement of maxillary glands Gelatinous 

 infiltration of subcutaneous and deeper structure* in various partt of 

 the body Stiffness, later dragging of the hind limbs whilst walking 

 Amelioration of symptoms ; considerable improvement in condition- 

 Further eruption of a pla^ut after intermission lasting 97 days. 



A bay Australian mare foaled in 1897, 15-2 J hands, purchased 20th Novem- 

 ber 1901, was cast from the Government Remount Dep6t, Karnal (No, 6418), 

 as a bad roarer, on 7th March 1903. Tho nnimal when received at Baieilly 

 was in good condition ; the temperature during the following 6 weeks always 

 remained between 37 and 38 C. 



April 10th, 1903. The mare was removed from the Plains Laboratory to 

 Muktesar, 7,600 feet above sea-level, and remained in the hills until Novem- 

 ber 10th, 1903, when she was again removed to Bareilly Dep6t. 



April 17th, 1903. Scarification with a fine needle was performed, involv- 

 ing 0'5 centimetre square of the mucous membrane of the right labium vaginae 

 in such a way as to draw little or no blood. As in the case of mare HI, 

 inoculated at the same time, a slight trace of blood was removed from a Dourine 

 plaque onKilngarth (T. B. English stallion) on the point of a scalpel and spread 

 over the scarified mucous-membrane and allowed to dry somewhat before the 

 labia were brought into apposition. 



Between the 17th and 26th April inclusive the animal fed well and exhibited 

 no symptoms of disease. The morning and evening temperatures varied 

 but a few points, the maximum and minimum readings being 37*9 and 37'1C., 

 the pulse and respirations averaging 40 and 13 respectively. 



On the 26th April, the 10th day following inoculation, the vaginal m. m. 

 presented a red colour and the small capillary vessels were injected. After 

 three days the right labium was tumified, and by the 15th day following 

 inoculation the vaginal m. m. became cedematous. both labia double the normal 

 dimensions, while the oedema had spread on either side of the vulva forming 

 folds in the tissues and over the perineum. Forty-eight hours later the oedema 

 extended downwards in the subcutaneous tissues of the right thigh and on palpa- 

 tion was found to be somewhat hot and semi-solid in consistency. The oedema- 

 tous condition of the vaginal m. m. persisted and eight months later was well 

 marked, but most of the other portions of the body invaded by the gelatinous 

 deposits from time to time were of an evanescent character, and daily exercise, 

 especially walking up hill, was usually sufficient to reduce aud at times to 

 totally disperse the swellings for short periods, especially those invading the 

 adder and under -surf ace of the abdomen. 



