JXX APPENDIX. 



On the 67th day the subrnaxillary glands were found to be slightly increased 

 in size, and three days later the first Dourine plaque appeared on the skin 

 of the right ribs. Between the 25th June and 8th October, the 70th to 175th 

 days following inoculation, eighteen plaques appeared in all, varying in size 

 and form, mostly urticanal in character. Trypanosomata were demonstrated 

 in blood drawn from the majority of these plaques. Then, on January 13th, 

 1904 (272nd day), after a long intermission lasting 97 days a further plaque 

 made its appearance. 



On the 14th July (89th day), some 18 hours after the eruption of plaque 

 No. 4, the mare showed symptoms of colic, which continued at intervals up to 

 6 P.M. when she aborted a four months foetus (trypanosoma not discovered 

 io foetal blood). The mare probably had been covered while running loose in 

 the Karnal Dep6t paddocks several weeks previous to being received at the 

 Bareilly Laboratory. On the 92nd day oedema of udder first appeared, followed 

 on the 113th by infiltration forward along the under-surface of the abdomen. 

 It was not until September 28th (165th day) that any symptoms of difficulty in 

 locomotion were noted. At first there was only stiffness of the right hiud 

 limb when walking, which however persisted until both limbs became affected. 

 On the 215th day after inoculation, the animal dragged the right limb and 

 two days later both limbs in walking. On the 223rd day the animal fell, and 

 was unable to rise without assistance, but when she was raised onto her legs, 

 again maintained the upright position. The right fore-limb became swollen, 

 and fluid appeared in several of the joints. 



The animal whose body weight six months before was 1,105 Ibs. now 

 rapidly lost flesh : there was especially marked atropy of the muscles of the 

 hips and loins, but the appetite never failed, and the body temperature main- 

 tained almost an even course between o7 and 38 C. 



During a considerable period there occurred alternately those augmentations 

 and diminutions in the size of the lips of the vulva, and in the oedematous 

 swellings in various regions of the body, but principally involving the external 

 genital organs and under-surface of the abdomen and limbs. 



January 12th, 1904, the 271st day after inoculation. The mare feeds well, 

 has improved considerably in condition, but there is still stiffness in the hind 

 limbs, the back giving way under pressure over the loins and when walking she 

 lifts her limbs carefully. The superficial inguinal and submaxillary glands are 

 still slightly enlarged, but not harder than normal. The vaginal mucus has 

 been constantly examined miscroscopieally up to the 12th January 1904 for the 

 presence of trypanosomata, and although on the 18th August petechise appeared 

 on the vaginal m. m., nevertheless no trypanosoma has been discovered in the 

 vaginal mucus unmixed with blood. 



January 13th (272nd day). A fresh plaque appeared on the right side of 

 the chest after an intermission lasting 97 days. 



