APPENDIX. 



mucous-membrane. Within 21 hours (5th) the maximum eruption of vesicles 

 had taken place, and some had already formed superficial ulcers, a form which 

 all assumed Inter, by the same oven in;;. 



Prom the 6th to the 13th the ulcers remained patent, probably ou account 

 of the muco-purulent discharge which appeared on the 10th, and persisted for 

 48 hours, when its place was taken by a sero-sanguinous discharge on the 12th 

 which made its escape from the vaginal orifice ; but it had totally disappeared 

 on the morning of the 31st. The process of healing sot in during the 14th, 

 and all ulcers had healed by the following day. The vaginal discharge persisted 

 in small quantity until the morning of the 1st August, when it assumed a 

 dirty colour and sticky character, just before disappearing on the 2nd August. 

 Twenty-six days later (28th) a little muco-purulent discharge escaped from the 

 vngina, but for a further period of many months, during which the animal 

 was kept under constant observation, no further return of it was observed. 



With regard to the general symptoms, on the morning of the 23rd July 

 1903, the 24th .day after the previous covering, the first cutaneous plaque 

 developed on the left hip, in the blood drawn from which, on microscopical 

 examination, the trypanosoma was discovered. Between the above-mentioned 

 date and the 29th December inclusive, a period of 1GO days, a succession of 

 plaques, ten in number, made their appearance singly, or in successive crops 

 within a few days. Thus, it may be observed on reference to Table XXIV, 

 that between the appearance of plaques No. 1 and No. 2, an interval 

 of 26 days elapsed, batween group II (consisting of Nos. 2, 3, 4, 5 plaques) 

 and the appearance of No. 6 a similar interval of 26 days elapsed. Between 

 group III (consisting of Nos. 6 and 7) and the appearance of No. 8 an 

 interval of 34 days took place. And between plaques Nos. 8 and 9 a period of 

 60 days transpired. Thus the length of the period of the intermissions (except 

 in the case of Nos. 1 and 2 which lasted in each instance 26 days) showed a 

 gradual increase during the persistence of the disease, viz., 26, 34, and 60 days 

 respectively. 



On the 13th August 1903, the trypanosoma was found for the first time in 

 the vaginal mucus, and, as may be seen in Table XXII, it continued to appear 

 at intervals throughout the period of observation lasting some months. 



The chief points of interest with regard to the other symptoms which 

 appeared in this animal between July 1903 and February 1904 may bo briefly 

 noted. An oedematons condition of the muoous-moaobrane of the vagina made 

 its appearance on the 3rd September and still persisted during the following 

 February, and on the 29th September the labia, which had previously remained 

 in apposition, now became persistently patent. The mare showed symptoms 

 of horsing at intervals, but excessive sexual excitement was only 

 present from the 6th to the 10th September inclusive. Considerable injec- 

 tion of the conjunctival capillaries became manifest on the 23rd September 

 and persisted until the morning of the 26th, when it disappeared. The 

 external inguinal glands, which up to the 20th November had shown no variation 

 from the normal condition, were on the date found to be slightly enlarged 

 and harder to the touch, but two months later they had regained their normal 

 proportions. (Edema ou the uuder-sxirface of the abdomen extending forward 



