XClV APPENDIX. 



DONKEY No. III. 



Inoculated Dourine in the Ass. 



A small entire donkey, bred in the Bareilly district, age 3| years, was 

 received at Muktesar in fair condition on July 31st, 1903. The body tempera- 

 ture of the animal scarcely varied between a maximum of 38 and a minimum 

 of 87'6 C. during the period of observation. 



August 4th, 1903, 3-30 P.M. The animal was uuboutaneously inoculated 

 with 15 c.c. of blood drawn from the jugular vein of the Arab stallion Mon* 

 arch during the interval which took place between the appearance of plaques 

 Nos. 69 and 70 on the early morning of 4th, and Nos. 71 and 72 on the morning 

 of the 6th August. 



August 5th 15th. A. diffused swelling appeared at the seat of inoculation 

 (6'x4"xO'5") which gradually decreased in extent during the 6th and 7th, and 

 had become absorbed by the morning of the 8th. From this latter date until 

 the evening of the 15th August, no symptoms were observed. The rectal tem- 

 perature between the 4th and 15th August varied between 38'3 and 36'9 C. 



August 16th. On the morning of this date, a swelling hard and warm on 

 palpation was observed at the seat of inoculation. Temperature 8 A.M. 37'6 C. 

 In the evening the temperature rose to 38-6 C., P. 80, R. 16. 



August 17th. The swelling as noted yesterday persisted, but the tempera- 

 ture had fallen to 38 C. in the morning, and rose but one point in the evening. 



During the two following days the swelling decreased, but thickening 

 remained at the seat of inoculation, and this condition persisted until the 6th of 

 the following month. 



September 5th. On this date, the 32nd day following inoculation, the 

 first plaque made its appearance on the skin over the left ribs. During the next 

 sixteen days nine other plaques followed, on various parts of the body. These 

 cutaneous patches persisted for varying periods., from a minimum of two days to 

 a maximum of forty. 



Blood drawn from these plaques was in its turn submitted to microscopical 

 examination, and the trypanosoma demonstrated in each respectively. Blood 

 drawn from the seat of inoculation revealed the presence of the trypanosoma 

 on the 16th August, the date of the re-appearance of the swelling. Specimens 

 of blood from the latter situation were made daily from 16th August to the 

 3rd September inclusive, in order to observe the varying forms exhibited by the 

 protozoon. The conclusions arrived at will be brought forward in the body of 

 the report, but it may be here stated that the mature trypanosoma was found 

 for several weeks in the sero-sanguinous fluid collected from the seat of in- 

 oculation. 



From the 22nd September 1903, until the end of February 1904, a period of 

 161 days, no more plaques or symptoms of Donrine have appeared. There has 

 been no swelling or oedema of the penis or sheath at any time, and it ia difficult 

 to imagine that the animal had previously been the subject of marked symptoms 

 of Do urine, 



