XCV1 APPENDIX. 



Temperature 38'5 C. which persisted during the next day. 4th A swelling 

 lias appeared at the seat of the injection, hot and red in colour, measuring 

 2'5"Xl'6''. 5th At 8 A.M. the swelling was found to be stationaiy, but at 

 1 A. ii. it began to increase rapidly in size and by the same evening its dimen- 

 sions were 9 7 x4'6''xO t 75"t The trypanosoma was observed in fluid examined 

 from the seat of inoculation. 6th The swelling had decreased aud continued 

 to do so until the morning of the 10th, when it is found to be absorbed. Slight 

 thickening of the skin persisted for some days longer, and was still present on 

 January 29th. February 29th, 1904, no other symptom of Dourine has 

 appeared up to date. 



No. IV Donkey. A small entire donkey, aged 7 years, bred in the 

 Bareilly district, was inoculated with 15 c.c. of blood drawn from the jugular 

 vein of the Arab stallion Yadgir on the 15th December 1903, on the 51st 

 day of an intermission lasting 53 days in all. The inoculated blood was collected 

 during the intermission between plaque No. 31 which appeared on the 25th 

 October and plaque No. 32 on the 18th December 1903. The eruption of 

 plaque No. 32 occurred 60 hours after the withdrawal of the collected blood. 

 On the 16th December a swelling appeared at the seat of inoculation 4'0''x 2'5'' 

 xO'5 7 which decreased during a period of four days. On the 21st the 

 thickening increased, and a tumour formed 2 < 5 ll 'X2 1 0' / xO > 25" at the seat of 

 inoculation, which continued until the 23rd, when it measured 3'0" X T25'' ; on 

 this date the swelling had become hard and tender on manipulation. From this 

 date it decreased in size and by the 29th December only a thickening of the 

 skin remained, February 29th, 1904 No other symptom of Dourine has 

 appeared up to date, with the exception of the eversion of the m. m. of the 

 meatus-urinarius. 



No. %I Pony. A country-bred pony, mare, aged, obtained from the 

 Bareilly district, was inoculated subcutaneously on the 16th December 1902 

 with 10 c.c. of blood drawn from the jugular vein of the Arab stallion 

 Shamshe? The blood was collected during an intermission which followed 

 the eruption of the last plaque in Lahore and the appearance of plaque No. 1 

 at Bareiliy on December 30th, 1902. 17th Swelling occurred at the seat of 

 inoculation and persisted for several days and then became absorbed. No 

 secondary swelling made its appearance, and no symptoms of Dourine followed. 

 On the 5th February 1903, the animal was re -inoculated with 2 c.c. of blood 

 obtained by incising plaque No. 3 on the L. hind limb of Shamsher on the 

 28th day after the stallion's arrival at Bareilly. Negative results followed. 



No. Ill Pony A country-bred pony mare, aged, obtained from the 

 Eareilly district, was inoculated subcataneously on the 16th December 1902 with 

 20 c.c. of blood drawn from the jugular vein of the Arab stallion Shamsher. 

 The blood was collected at the same time aud under the same conditions as in 

 the case of pony No. II. Primary swelling followed at the seat of injection' 

 but the results were negative. On the 5th February 1903, re-inoculation was 

 carried out with 20 c. c. of blood drawn from jugular vein of Shamsher as 

 on the previous occasion. The blood collected from the Arab stallion on the 

 first occasion was during the persistence of an intermissiou and 14 days pro* 

 rious to tte eruption of the next plaque. That utilized for the second operation 



