APPENDIX. XCVli 



was drawn from the general circulation of the affected stallion, only a few 

 hours after the appearance of plaque No. 9 (2'0 inches in diameter) in the blood 

 of which the trypunosoma was discovered. Negative results also followed the 

 second inoculation. 



No. VI Pony. A country-bred pony, mare, aged 8 years, obtained from 

 the Bareilly district, was inoculated subcutaneous ly on the 16th December 

 1903 with 21 c.c. of blood drawn from the jugular vein of the Arab stallion 

 Shamsher. The blood was collected during the intermission of eighty days 

 which followed the eruption of plaque No. 19 on the 2yth September and the 

 appearance of plaque No. 18 on December 18th, 1903. The eruption of the 

 latter plaque occurred 36 to 40 hours after the operation for bleeding. On the 

 17th, the swelling at the seat of inoculation measured 7'5' X Ti/'x 0*5" and was 

 soft in consistency. By the 21st the swelling had become absorbed. Between 

 the 22nd December 1903 and February 29th, 1904, the animal fed well and no 

 further symptom of Donrine had been developed. 



No. VII Pony. A country-bred pony, mare, aged 3 \ years, obtained from 

 the Bareilly district, was inoculated subcutaneously on the 16th December 1903, 

 with 27 c.c. of blood drawn from the jugular vein of the thorough-bred English 

 stallion Kilngarth. The blood was collected during the intermission of ten 

 days which followed the eruption of plaque No. 39 on the llth December and 

 the appearance of plaque No. 40 on the 22nd December 1903, six days before 

 plaque No. 40 became manifest. On the 17th the swelling at the seat of 

 inoculation measured 4'5"x I'O'xO'iS 7 and was soft in consistency. The 

 swelling gradually decreased in the next few days and had totally disappeared 

 by the 21st. Between the 22nd December 1903 and February 29th, 1901, the 

 animal showed no signs of indisposition, and no symptoms of Dourine were 

 developed. 



Bull No. 124. Plains animal, age 2 years, mixed breed, from the Bareilly 

 district. Inoculated mbcutaneously with 20 c.c. of blood drawn from the 

 jugular vein of Arab stallion Monarch on the afternoon of the 9th Febru- 

 ary 1904, some hours after the eruption of plaque No. 83, on the 283rd day 

 after covering mare III. No swelling or oedema followed at the seat of injec- 

 tion and up to the 29th February 1904 no symptoms had manifested them- 

 selves. Temperature remained within normal limits. 



Dog No. I. A pariah puppy, aged 4 months, was inoculated subcutan- 

 eously with 0'5 c.c. blood drawn from plaque No. 2 Arab stallion Shamsher 

 on the 13th January 1903, The trypanosorna was found in the injected blood. 

 Result negative. He-inoculation was performed on the 5th February, when 10 

 c.c. of blood drawn from the jugular vein of Shamsher was injected. 

 Plaque No. 3 had only appeared a few hours before the withdrawal of blood. 

 March 3rd, 1903. Inoculated subcutaneously for the third time with To c c. 

 of sero-sanguinous fluid collected from the oedema on the under-surface of the 

 abdomen of Shamsher. 



Dcg No. II. -A pariah puppy, aged 4 months, was inoculated snbcntan* 

 eously with 5 o.c. of blood drawn from the jugular vein of Shamsher on 

 the 13th January 1903, during the intermission which followed the eruption of 

 plaque No. 2 and the appearance of No. 3. Result negative. Re-inoculation 



