APPENDIX. 



was performed on the 6th February, when 0'45 c.c. o hlood collected 

 from the freshly formed plaque No. 4 of Shamsher was injected subcutan- 

 eously. Plaque No. 3 had appeared 24 hours previously and No. 4 only a few 

 hours before the operation. The animal was inoculated for the third time with 

 I/O c.c. of hlood collected from plaque No. 5 on the 17th February 1903. 

 The animal was inoculated for the fourth time with blood drawn from plaque 

 No. 7 on the 2nd March 1903. Negative results during the next three months. 



Dog No. III. A. yonng pariah dog was inoculated subcutaneousl y on 27th 

 May 1903 with 10 c.c. of blood drawn from the jugular vein of thorough- 

 bred English stallion Kilngarth. Between the 21st and 28th May a succes- 

 sion of 6 plaques made their appearance at intervals of one to two days, the 

 trypanosomata were found in blood drawn from the plaques on the dates of 

 their respective appearance ; it was considered more than probable that the 

 protozoon would be present in some form in the blood of the general circula- 

 tion. This animal was kept under observation for 6 months and daily examin- 

 ations of the blood made, but neither cutaneous symptoms nor trypanosomata 

 manifested themselves. Body weight on 27th May 1903 29 Ibs., and on 5th 

 August 1903 34 Ibs. 



Dog No. IV. A young pariah dog was inoculated on 27th May 1903 in 

 the anterior chamber of the eye, with 4 minims of blood drawn from plaque 

 No. 12 (c) Kilngarth, a specimen of which, on microscopical examination, 

 was found to contain the Dourine trypanosoma in some numbers. The eyes 

 passed through the usual phases, but eventually the cornea cleared, but no 

 symptoms of Dourine were observed during a period of 6| months, although 

 the blood was submitted to microscopical examination daily. The morning and 

 evening temperatures of the animal during the same period, with few excep- 

 tions, continued an even course between 38 and 39 C. Body weight on 27th 

 May 1903 26 Ibs., and on 7th August 1903 34 Ibs. 



Babbit No. /.Body weight 950 grammes. On 27th May 1903, inocu- 

 lated subcutan eously with 4 c.c. of blood drawn from the general circulation of 

 Kilngarth (jugular vein). No symptoms of the disease supervened, and the 

 trypanosoma was never discovered, although the blood was examined daily until 

 26th September 1903. Body weight on 22nd September 1903 1,920 grammes. 

 No symptoms of Dourine developed. 



Rabbit No. II. Body weight on 27th May 1903 1,440 grammes. Inocu- 

 lated in the anterior chamber of the eye with 0'2 c.o. of blood drawn from plaque 

 No. 12 (c) Kilngarth. Trypanosoma present. No rise in temperature followed 

 the injection and the cornea cleared after some days and became transparent 

 again. On the morning of the 16th July the temperature was found to have 

 risen from 39'3 C., the previous evening's record, to 41-3 C. This temperature 

 Was maintained during a period of 36 hours, when it fell, and on the morning 

 of the 19th registered 39-3* 0. Death took place at 10-30 A.M. on the 18th 

 July. Body weight on 27th May 1903 1,440 grammes, on 18th July 1903 

 1,530 grammes. 



Guinea-pig* Bo&j weight 600 grammes, was inoculated subcutaneously 

 with 3 drops of blood containing the Dourine trypanosoma, obtained from 

 plaque No. 12 (c) Kilngarth. On the 27th May 1903, the temperature pre- 



