464 NORMAL HISTOLOGY AND ORGANOGRAPHY. 



Review of Preparing Tissues. 



1 . Fixing and hardening 



2. Washing. 



3. Dehydrating. 



4. Imbedding in celloidin. 



5. Mounting on block and evaporation of 



ether. 



6. Imbedding in paraffin. 



7. Cutting sections. 



8. Staining celloidin sections. 



9. Staining paraffin sections. 



The following is a brief outline of the tissues to be 

 prepared for a laboratory course accompanying the 

 text. Special technique is cited whenever indicated, 

 otherwise standard laboratory methods may be em- 

 ployed. This outline is abbreviated and should be 

 expanded according to the skill of the instructor or 

 student and according to the laboratory equipment. 



Mitosis. 



1 . Growing point of onion or lily root tip, hardened 

 in Flemming or corrosive sublimate, and cut in longi- 

 tudinal section. 



2. Testicle of grasshopper taken in June, or ova, 

 or skin stripped from tail of growing tadpoles. Iron 

 nematoxylin stain is excellent. 



Epithelium. 



i Isolated epithelial cells from intestine, trachea 

 and bladder (see page 457). 



2. Epithelium exfoliated from skin of frog (pieces 

 gathered from water where frogs are kept). 



3. Fresh cells scraped from mucous surface of cheek. 



4. Sections of intestine, cornea of the eye, and skin. 



5. Endothelial cells of mesentery. 



