472 NORMAL HISTOLOGY AND ORGANOGRAPHY. 



3. Spinal cord. The cord of the horse or ox is 

 very satisfactory. 

 Brain. 



1. Sections of cerebral cortex. 



2. Sections of cerebellar cortex cut across the 

 folds. 



3. Sections of closed and open medulla. 



4. Sections of the pons. 



Sections of the medulla and pons should be cut 

 thick and stained with Pal-Weigert method. 

 Celloidin method of imbedding is preferable. 

 The Eye. 



1. Sections of the whole eye imbedded in celloidin. 



2. Sections of the cornea. 



3. Sections of the retina. 



4. Sections of the eyelid. 

 The Internal Ear. 



Sections of the cochlea must be decalcified. 

 Cochlea of a young kitten is very satisfactory. 



STANDARD FIXING SOLUTIONS. 

 Carney's Acetic-alcohol Mixture. 



Glacial acetic acid 1 part. 



Absolute alcohol 3 parts. 



Pieces one centimeter thick are fixed in one-half to 

 one hour. The after-treatment is with absolute 

 alcohol. 



Osmic Acid. One-half to one per cent, aqueous 

 solution. 



Fix for three to twenty-four hours and then wash 

 thoroughly in running water. 



