474 NORMAL HISTOLOGY AND ORGANOGRAPHY. 



Fix for several hours and wash thoroughly in running 

 water. 



Chromic Acid. Aqueous solution to i per cent. 

 Small pieces are fixed for twenty-four hours. Wash 

 in running water and pass through the ascending 

 grades of alcohol, preferably in the dark. 



Miiller's Fluid 



Potassium bichromate 2.5 gm. 



Sodium sulphate 1.0 gm. 



Water 100 c.c. 



Fix for several weeks, preferably in the dark. At 

 first the fluid should be changed daily. Wash in 

 running water for twenty-four hours and place 

 directly in 70 per cent, alcohol. Dehydrate prefer- 

 ably in the dark. 



Zenker's Fluid. 



Potassium bichromate 2.5 gm. 



Sodium sulphate i.o gm. 



Corrosive sublimate 5.0 gm. 



Glacial acetic acid 5.0 gm. 



Water 100 gm. 



It is better to add the acetic acid just before using 

 and not to add it to the stock solution. Fix tissues 

 in this fluid for six to twenty-four hours. Wash in 

 running water twenty-four hours and transfer to 

 alcohol, using the grades. Sublimate crystals are 

 removed by iodized alcohol as with corrosive subli- 

 mate solutions. 

 Formalin. 



Formalin (40 per cent. Formal- 

 dehyde) 5 to 10 parts. 



90 parts. 



Water. 



Small pieces are fixed in twelve to twenty-four hours. 

 As sections do not stain well after this fixation, it 



