476 NORMAL HISTOLOGY AND ORGANOGRAPHY. 



Alum-carmin (Grenacher). 



Alum solution, 3 per cent, to 5 



per cent 100 c.c. 



Carmin 1 to 5 gm. 



Boil for fifteen minutes, cool and filter. Add enough 

 water to replace that lost by boiling. Wash the 

 sections in water after staining. 



Bohmer's Hematoxylin. 



Hematoxylin 1 gm. 



Alcohol, absolute 10 c.c. 



Potassium alum 10 c.c. 



Distilled water 200 c.c. 



Dissolve the hematoxylin in alcohol and the alum in 

 water. Add the first to the second solution while 

 continually stirring. Allow this preparation to 

 stand in an open jar for two weeks, to ripen, when 

 the color will change from violet to blue. After 

 filtering the stain is ready. 



Delafield's Hematoxylin. 



Hematoxylin crystals 4 gm. 



Absolute alcohol 25 c.c. 



Ammonium alum, sat. aq. sol. .400 c.c. 



Alcohol 95 per cent 100 c.c. 



Glycerin 100 c.c. 



Dissolve the hematoxylin in absolute alcohol and add 

 the alum solution. Place in open vessel for four 

 days, filter, and add the 95 per cent, alcohol and 

 glycerin. In a few days filter again. 



Ehrlich's Hematoxylin. 



Hematoxylin crystals 2 gm. 



Absolute alcohol 60 c.c. 



Glycerin 1 saturated with 60 c.c. 



Distilled water I ammonia-alum 60 c.c. 



Glacial acetic acid 3 c.c. 



Expose to light for a long time. It is ready for use 

 when it acquires a deep red color. 



