VI CONTENTS. 



CHAPTER VI. 



PAGE 



Preparation of media Bouillon, gelatin, agar-agar, 

 potato, blood-serum, etc 59-76 



CHAPTER VII. 



Preparation of the tubes, flasks, etc., in which the media 

 are to be preserved . 77-80 



CHAPTER VIII. 



Technique of making plates Esmarcli tubes, Petri 

 plates, etc 81-90 



CHAPTER IX. 



The incubator used in bacteriological work (las-pivs>- 

 ure regulator Thermo-regulator The form of burner 

 employed in heating the incubator 91-98 



CHAPTER X. 



The study of colonies Their naked -eye peculiarities 

 and their appearance under different conditions Differ- 

 ences in the structure of colonies from different species of 

 bacteria Stab cultures Slant cultures .... 99-103 



CHAPTER XI. 



Systematic study of an organism Steps necessary in 

 identifying an organism as a definite species . . . 104-121 



C H A P T E R XII. 



Methods of staining Solutions employed Preparation 

 and staining of cover-slips Preparation of tissues for 

 section-cutting Staining of tissues Special staining 

 methods 122-150 



CHAPTER XIII. 



Inoculation of animals Subcutaneous inoculation 

 Intra-venous injection 151-158 



