80 BACTERIOLOGY. 



each of three successive days. During the intervening 

 days they may be kept at the ordinary room temperature. 



When sterilization is complete, and the medium in 

 the tubes is still liquid, some of them may be placed in a 

 slanting position, at an angle of about ten degrees with 

 the surface on which they rest, and the medium allowed 

 to solidify in this position. These are for the so-called 

 slant cultures. The balance may solidify in the erect 

 position ; these serve for the plate cultures. 



For Esmarch tubes not more than 5 c.c. of material 

 should be placed in each tube, as more than this renders 

 the rolling difficult and irregular. 



