SCHEME FOR STUDY OF AN ORGANISM. 165 



what way are the flagellae given off from the body of the 

 organism ? 



16. Does it produce gas-bubbles in ordinary agar- 

 agar or gelatin ? 



17. Does it produce gas-bubbles in agar-agar or gel- 

 atin to which 1 to 2 per cent, grape sugar has been 

 added ? 



18. Does it produce indol? Is the indol accom- 

 panied by a coincident production of nitrites ? 



19. At what temperature does it grow most luxu- 

 riantly ? 



20. What is the lowest temperature at which it 

 grows? 



21. Is it aerobic, anaerobic, or facultative in its rela- 

 tions to oxygen? 



22. What are its staining peculiarities ? 



23. Will it withstand drying ? 



24. At what temperature is it killed by heat both 

 by steam and the hot-air method ? 



25. Is it pathogenic when introduced either subcu- 

 taneously or directly into the circulation of animals? 

 If so, for which animals? Do any of the animals used 

 for this work possess natural immunity against infection 

 by this organism ? 



26. What are the histological appearances seen in the 

 tissues of animals for which this organism is pathogenic ? 



