SPUTUM SEPTICAEMIA. 197 



surface of the gelatin. The growth is very slow and no 

 liquefaction of the gelatin occurs. 



If grown in slant or stab cultures, the surface devel- 

 opment is very limited ; along the needle track very 

 small, whitish granules appear. This organism is con- 

 spicuous for the rapidity with which it loses its patho- 

 genic properties and the fact that after a very few gen- 

 erations it can no longer be caused to grow. 



On agar-agar the colonies are almost transparent; they 

 are more or less glistening and very delicate in their 

 structure. 



On blood-serum the growth is more marked, though 

 still extremely feeble. Here it appears as a very deli- 

 cate film, consisting of fine points growing closely side 

 by side. 



A growth on potato has not been observed. 



The organism is not motile. 



It grows best at a temperature between 35 C.-38 C. 



Under 24 C. no growth has been observed, and from 

 42 C. on, the development is checked. 



Under most favorable conditions the growth is very 

 slow. It grows as well without as with oxygen. It is, 

 therefore, one of the facultative anaerobic forms. 



The most successful efforts at the cultivation of this 

 organism are those seen when the agar-gelatin mixture 

 of Guarniari is employed. (See this medium.) 



It may be stained with the ordinary aniline staining 

 reagents. For demonstration of the capsule the method 

 of Gram gives the best results. (See Staiuings.) 



6. SEPTICAEMIA OF THE MICROCOCCUS TETRAGENUS. 



Should the death of the animal not occur within 

 the first twenty -eight to thirty hours after inoculation, 



