GEORGE: w. GOLKR, M. D V AND F. R. EILENGKR, PH. G. 163 



selling- that milk in the city until he has a clean bill of health 

 for all his herds from the State Department of Agriculture. In 

 this way the city not only helps to clean up its manifestly tuber- 

 culous herds, but secures the most positive kind of evidence with 

 which to ask for a' strong, immediate, well enforced ordinance 

 requiring the testing of all cattle. 1 



This brief note is only intended to record results obtained in 

 the work of roughly testing the milk of 185 retailers supplying 

 the city of Rochester with milk for the purpose of securing evi- 

 dence upon which to base a demand for an annual tuberculin 

 test of all the cattle supplying to the city. 



In the beginning of our experimental work we depended upon 

 naked eye evidence in the organs of those animals dying after 

 acute infection had passed, or, who, living two months after the 

 injection were upon autopsy found with marked lesions of tuber- 

 culous disease within their bodies. In the first hundred animals 

 we depended upon these post-mortem naked eye lesions, but 

 later, owing to the test suggested by Anderson, 2 we were able 

 to determine more clearly and closely the animals reacting by 

 injecting them with 2 c. c. of crude tuberculin after they had 

 lived two months. 



We have in all, in Rochester, 185 licensed retail dealers. 

 Taking a sample from each one of these dealers, we injected 

 one animal with centrifugalized cream, another with centrifuga- 

 lized sediment from the same sample. Where both animals died 

 of acute infection duplicate samples were obtained and injected 

 other animals. All told we used 242 samples and 484 animals. 

 Of these the first 100 animals used exclusive of those dying of 

 acute infections, 5 or 5 per cent, were found with marked naked 

 eye tuberculous lesions. Of the whole number of pigs injected 

 285 received injections of 5 c. c. of sediment in milk, and of 

 these 61 or 21.4 per cent, died shortly after inoculation as a 

 result of acute infections. Of the whole number of animals 

 injected 199 received 5 c. c. of cream, of which number 86 or 

 43.2 per cent, died shortly after inoculation. 237 pigs were in- 

 jected with 2 c. c. of crude tuberculin, of which 30 reacted or 

 12.6 per cent. Of these animals 18 reacted that had been in- 

 jected with sediment or 7.59 per cent. ; 12 that had been injected 

 with cream or 5.06 per cent. 



As a result of this work 41 retailers supplied by 28 producers 

 had 757 cattle tested. 671 cattle were tested through the efforts 

 of the Chief Milk Inspector, Mr. W. O. Marshall. 200 cattle 

 were tested because the owners voluntarily asked for the test, 



iFor the details of this plan, both the laboratory work and that of the 

 New York State Department of Agriculture in testing cattle and reimbursing 

 owners of tuberculous cattle, the reader is referred to my preliminary report 

 in the Albany Medical Annals, Feb. 1910. 



2Journal Infectious Diseases, March, 1908. 



