INFECTIOUS DISEASES 199 



of the first four pigs should die of cholera during the 

 time of the test. 



The serum that has been tested and in which the two 

 check pigs have died of cholera and the first six pigs have 

 shown no visible illness nor any constant rise of tem- 

 perature is ready for mixing and bottling, after which it 

 is ready for use. The laboratory for mixing and bottling 

 must be properly constructed in order that contamination 

 of the serum may be reduced to a minimum and the neces- 

 sary equipment, such as sterilizers, must be provided. 



For mixing the serum a container must be selected 

 that can be sterilized and from which the serum can be 

 bottled. 



After thoroughly mixing the serum constituting a 

 series it is withdrawn into sterile bottles, the size of which 

 will be determined by the demand. The bottles are 

 properly sealed, labeled, and wrapped, and until needed 

 put back in storage at a temperature not to exceed 50 

 degrees Fahrenheit. 



The foregoing description is the general method of 

 production of anti-hog-cholera serum. Many little fac- 

 tors that are of considerable importance have not been 

 enumerated. The accompanying illustrations may con- 

 vey some idea of the problems of the production of this 

 product. The production of, anti-hog-cholera serum has 

 become of such vast importance to the swine industry 

 of the country that Congress has provided means for its 

 regulations in order that a potent serum may be obtained. 



The following report of the committee on Uniform 

 Methods for the Control of Hog Cholera was adopted 

 by the United States Live Stock Sanitary Association 

 in Chicago, December, 1913, and is a brief statement of 

 the requirements for serum production: 



Serum Production 



Location of buildings. 



Location to be such that surroundings will not interfere with sanitary 

 production and that their operation will not contaminate Biurouadinjf 



