01 THE TWO MIDDLE ;I:KM i. \VERS. 141 



of tin- middle IT- rm-la\vr> d< ia<-h them- Ives from tin- adjacent (11- 

 lavers at th.' >ili-s where tin ir ingrowth took place, and at the same 

 time tin- halves of the permanent entoderm grow together, wh-T- l>y 

 the dorsal closure of the intestine is effected. 



In \ie\vofthesefactsthere is only one explanation at which we 

 can arrive. If it is certain that the middle germ-layers do not 

 ari>e Iv a iiim in [<'<> 1'rom either of the primary germ-layers, 

 then their gradual sj>r< ading out from a definite region of the germ 

 (an result only from an ingrowth of cells, which <>m those 



places where a connection with other cell-layers has been demon- 

 strated. The middle germ-layers draw the principal material for 

 their growth from cells which, at the blastopore or at the primitive 

 groove, migrate between the two primary germ-layers. 



But this immigration of cells may be interpreted as a process of 

 infolding of the primary germ-layers, as in the case of Amphioxus. 

 In the method of the infolding there exists, it is true, one very 

 striking and apparently important difference between Amphi' 

 and the remaining Vertebrates. In Amphioxus the middle germ- 

 layer arises as a hollow sac, by means of the folding of the inner 

 germ-layer in the remaining Vertebrates as a solid mass of cells. 

 This undeniable difference is, however, easily explained in the 

 following manner : In the solid fundaments of the middle germ- 

 layer a cavity is wanting, because the cellular walls of the sac are 

 from the beginning firmly pressed together, in consequence of the 

 yolk-mass which fills the coelenteron. In addition to other striking 

 ements with the conditions in Amphioxus lanceolatus, there are 

 three pointsof viewwhich in particular commend this interpretation : 



(1) In all vertebrated animals there early arises in the middle 

 germ-layer a fissure, which is surrounded by cells, often cubical or 

 cylindrical, having an epithelial arrangement. The parietal and 

 ;al layers then take the form of epithelial lamellae, as is to be 

 >eeii in an especially striking manner in the case of the Selachii at 

 a very eanly stage of development. (2) From these epithelial layers 

 there arise in the adult genuine epithelial membranes, like the 

 ciliated peritoneal epithelium of many Vertebrates, and. in addition, 

 glands that in many respects resemble the glands derived from 

 piihelial membranes [of the other germ -lay. i>| (kidney, ; 

 ovary). (3) The objection that the middle germ -layer of Verte- 

 brates arises as a single cell-mass, and therefore cannot be equi- 

 valent to two layers of epithelium, loses its weight with every one 

 who knows the numerous analogous phenomena of development 



