64 



INFECTION, PHAGOCYTOSIS, OPSONINS 



3. Get blood from three healthy horses. Separate the serum from 

 the corpuscles in each case by centrifuging or coagulation. Mix the 

 three sera in equal proportions. This mixture, in work with opsonins, 

 is called the pool. 



4. Get blood from the sick horse, and after coagulation, separate 

 the serum from the corpuscles. 



FIG. 27 



After the pipette has been prepared, its contents are repeatedly expelled and drawn up again 

 in order to mix the three constituents well. (Miller.) 



FIG. 28 



After the pipette has been in the incubator, the contents are blown on a slide and the drop is 



drawn out. (Miller.) 



5. Mix in equal amounts the washed white blood corpuscle 

 emulsion with the three-serum mixture from healthy horses (the 

 pool), and with the centrifuged-heated culture (emulsion) of the 

 Staphylococcus pyogenes aureus. 



6. Mix in another small pipette equal amounts of the white blood 

 corpuscle emulsion of the serum of the sick horse and of the cen- 

 trifuged heated bouillon culture of the Staphylococcus pyogenes 

 aureus. 



