122 STAINING OF BACTERIA 



QUESTIONS. 



1. What is an anilin stain? What three types are distinguished? 



2. Name the three anilin stains most commonly employed in working with 

 bacteria. How are the solutions of these stains prepared? 



3. Describe the method of staining a cover-glass preparation of pus for 

 bacteria. 



4. Describe method of preparing and staining a cover-glass specimen from a 

 pure culture. 



5. What precautionary measure should be observed when staining live patho- 

 genic bacteria? 



6. Why is it particularly dangerous to work with live cultures of the glanders 

 bacillus? 



7. Give formula for Loeffler's alkaline methylene blue. 



8. Give formula for (a) anilin-water gentian violet; (6) Gram's decolorizing 

 fluid. 



9. Describe Gram's method of staining and decolorizing bacteria. 



10. Name some bacteria which stain by Gram's method and some which do 

 not. 



11. Give formula for Ziehl's carbol-fuchsin. 



12. Give Johne's method for staining the capsules of bacteria. 



13. Describe one of the methods for staining spores. 



14. Give one of the methods for staining flagella. 



15. Describe the method of staining the polar bodies. 



16. Describe the method of using Wright's stain on a cover-glass preparation 

 of blood or pus. 



17. Describe the use of Giemsa's stain for demonstrating Spirochsetse pallida. 



18. Describe the celloidin -embedding method. 



19. Describe the paraffin-embedding method. 



20. Describe Mallory's eosin-methylene-blue staining method. 



21. Describe the Gram-Weigert staining method for tissues. 



22. Describe Levaditti's silvering method. 



NOTE. The student need not spend much time in learning by heart formulae 

 for stains and staining methods; these are best acquired by practice. 



