202 WOUND INFECTION AND PYOGENIC BACTERIA 



Resistance. The Bacillus pyocyaneus is quite resistant. It cannot 

 be readily killed by drying out, and its behavior toward antiseptics 

 and germicides is similar to that of the pyogenic staphylococci. 



QUESTIONS. 



1. Explain the term pyogenic bacteria. 



2. Which are the most common pyogenic microorganisms? 



3. Where are they found? 



4. What does the term ubiquitous mean? 



5. Are pyogenic bacteria most commonly found as parasites? 



6. What is meant by wound healing by primary intention? 



7. What is meant by a local; what by a general pyogenic infection? 



8. What is a septicemia? 



9. What are multiple metastatic bacterial emboli? 



10. What is a septicopyemia? 



11. What is a mixed infection? 



12. What is a sapremia? 



13. Name- the three different varieties of the common pus-forming cocci? 



14. Name a number of diseases caused by the pyogenic staphylococci. 



15. Describe the Staphylococcus pyogenes aureus in a hanging drop and in a 

 stained cover-glass preparation. 



16. How does this coccus act when stained by Gram's method? Describe 

 this staining method. 



17. How does the Staphylococcus pyogenes generally present itself in pus? 



18. At what temperature does this coccus grow? What is its optimum temper- 

 ature? What is its action toward free oxygen? 



19. Describe a bouillon culture and a gelatin stab culture of the Staphy- 

 lococcus pyogenes aureus. 



20. What is meant by the hemolytic property of the Staphylococcus pyogenes? 



21. What is leucocidin? 



22. What is amyloid material or substance? 



23. Discuss the resistance of the Staphylococcus pyogenes toward physical 

 and chemical agencies. 



24. What is the effect of vaccine therapy in chronic Staphylococcus infection? 

 Describe the principle and details of Staphylococcus vaccine therapy. 



25. Give the morphologic features of the Streptococcus pyogenes. 



26. What is the difference between Streptococcus pyogenes longus and Strepto- 

 coccus pyogenes brevis? 



27. What are the staining properties of the Streptococcus pyogenes? 



28. Name some Gram-negative streptococci. 



-29. What kind of media are best adapted to the growth of Streptococcus 

 pyogenes? 



30. What effect have peptone and sugar upon the growth of the Streptococcus 

 pyogenes? 



31. What culture medium is best for preserving the virulency of Streptococcus 

 pyogenes? 



32. Describe the colonies of the streptococcus on agar plates. 



33. Under what conditions does the streptococcus liquefy gelatin? 



34. What is its optimum temperature? What is its relation to free oxygen? 



35. Discuss the resistance of the streptococcus. 



36. Describe the occurrence and pathogenesis of the Bacillus pyocyaneus. 

 Why is it called pyocyaneus? 



37. Describe the morphologic features of this bacillus. 



38. Does it belong to the amphitrichse or peritrichae? 



39. What effect does an intraperitoneal injection of the bacillus into a male 

 guinea-pig sometimes have? Why is it important to remember this? 



40. Describe a gelatin plate and a gelatin stick culture of the organism. 



41. Describe a bouillon culture. 



42. What is meant by auto lysis and an autolytic ferment secreted by the 

 Bacillus pyocyaneus? 



43. What are the properties of the two pigments formed by the Bacillus 

 pyocyaneus? 



44. What kind of toxins does this bacillus form? 



45. Discuss the resistance of the organism. 



