QUESTIONS 527 



QUESTIONS 



1. What apparatus is required for the simple chemical manipulations employed 

 in elementary bacteriology? 



2. What is a burette, a pipette, a beaker, an evaporating dish? 



3. What is meant by the gravimetric, what by the volumetric method of 

 quantitative chemical analysis? 



4. What is a normal solution ? What a decinormal, centinormal solution ? 



5. What is the atomic weight of an elementary body? What is the molecular 

 weight of a compound body ? 



6. What is the atomic weight of N, O, C, and S? 



7. What is the molecular weight of caustic soda? 



8. What is the procedure in preparing a set of acid and alkaline normal 

 solutions for use in the standardization of the common culture media? 



9. How is the acid standardized against the alkaline solution? Is it easy 

 to prepare them so that 100 c.c. of the acid normal solution will exactly neutralize 

 100 c.c. of the alkaline normal solution? 



10. If they are not exactly balanced what can you do to correct the result ? 



11. What is a titration? 



12. What is an indicator? 



13. Describe the preparation of the following indicators: Dimethylamidoazo- 

 benzol, litmus, rosolic acid, phenolphthalein. 



14. Give the formula for Fehling's standard solution. 



15. What is an empirical standard solution? 



16. Describe the use of Fehling's standard solution in the quantitative esti- 

 mation of sugar in a fluid. 



17. How much of the following sugars does it take to reduce all of the copper 

 hydrate contained in 20 c.c. of Fehling's solution? Glucose, maltose, lactose? 



18. Describe the indicator used in connection with Fehling's solution. 



19. How does this indicator work ? 



