232 The Identification of Species 



* Liquefaction of the gelatin takes place slowly. Ferment 

 urea, with strong production of ammonia. URO-BACILLUS 

 GROUP OF MIQUEL. 

 ** Gelatin liquefied rather quickly. 



t Potato cultures rugose. POTATO BACILLUS GROUP. 

 tf Potato cultures not distinctly rugose. B. SUBTILIS GROUP. 

 b. Gelatin not liquefied. B. SOLI GROUP. 



2. Rods spindle-shaped at sporulation. B. LICHENIFORMIS GROUP. 



3. Rods clavate at sporulation. B. SUBLANATUS GROUP. 

 B. Obligate anaerobic. 



1. Rods not swollen at sporulation. MALIGNANT EDEMA GROUP. 



2. Rods spindle-shaped at sporulation. CLOSTRIDIUM GROUP. 



3. Rods clavate-capitate at sporulation. TETANUS GROUP. 



PSEUDOMONAS (Migula) 



I. Cells colorless, without a red-colored plasma and without sulphur granules. 



A. Grow in ordinary culture-media. 



i. Without endospores. 



a. Aerobic and facultative anaerobic. 



* Without pigment. 



t Gelatin not liquefied. 



Gas generated in glucose bouillon. Ps. MONADIFORMIS 



GROUP. 



No gas generated in glucose bouillon. Ps. AMBIGUA GROUP. 

 ft Gelatin liquefied. 



Gas generated in glucose bouillon. Ps. COADUNATA GROUP. 

 No gas generated in glucose bouillon. Ps. FAIRMONTENSIS 

 GROUP. 



* Produce pigment on gelatin or agar. 



t Pigment yellowish. 



Gelatin liquefied. Ps. OCHRACEA GROUP. 

 Gelatin not liquefied. Ps. TURCOSA GROUP. 

 ft Pigment blue-violet. 



Gelatin liquefied. Ps. JANTHINA GROUP. 

 Gelatin not liquefied. Ps. BEROLINENSIS GROUP. 

 ** Produce a greenish-bluish fluorescence in culture-media. 



t Gelatin liquefied. Ps. PYOCYANEA GROUP. 

 ft Gelatin not liquefied. Ps. SYNCYANEA GROUP. 

 2. With endospores, aerobic and facultative anaerobic. 



a. Non-chromogenic. 



* Rods not swollen at sporulation. Ps. ROSEA GROUP. 

 ** Rods swollen at one end at sporulation. Ps. TROMMEL- 



SCHLAGER GROUP. 



b. Produce a greenish-bluish fluorescence in culture-media. 



* Gelatin liquefied. Ps. VIRIDESCENS GROUP. 

 ** Gelatin not liquefied. Ps. UNDULATA GROUP. 



B. Do not grow in nutrient gelatin or other organic media. NITRIMONAS 



GROUP. 



II. Cell plasma with a reddish tint, also with sulphur granules. CHROMATIUM 

 GROUP. 



MICROSPIRA (Migula) 



I. Cultures show a bluish-silvery phosphorescence. PHOSPHORESCENT GROUP. 

 II. Cultures not phosphorescent. 



A. Gelatin liquefied. 



1. Cultures show the nitro-indol reaction. 



a. Very pathogenic to pigeons. MSP. METSCHNIKOVI GROUP. 



b. Not distinctly pathogenic to pigeons. CHOLERA GROUP. 



2. Nitro-indol reaction negative or very weak, at least after twenty- 



four hours. CHOLERA NOSTRAS GROUP. 



B. Gelatin not liquefied or only slightly so. MSP. SAPROPHILA GROUP. 



MYCOBACTERIUM (Lehmann-Neumann) 



I. Stain with basic anilin dyes, and easily decolorized by mineral acids when 

 stained with carbol-fuchsin. 



