Zur Nedden's Bacillus 409 



serum is almost immediately liquefied, so that the growing colonies 

 appear to be sinking into the medium after thirty-six hours. The 

 entire tube of medium may eventually be liquefied. 



Upon agar-agar containing serum, grayish-white colonies of 

 small size, resembling colonies of gonococci, are formed. Growth 

 is slow. Bouillon is slowly clouded. 



Pathogenesis. The pathogenic and specific nature of the diplo- 

 bacillus was made clear by Morax, who produced the disease in 

 man by placing a pure culture upon the human conjunctiva. 



ZUR NEDDEN'S BACILLUS 



This bacillus was the only organism that Haupt* was able to 

 isolate from a neuroparalytic with confluent peripheral ulcera- 



, 



Fig. 149. The Morax-Axenfeld diplobacillus of conjunctivitis. Magnified 

 1000 diameters (Rymowitsch and Matschinsky). 



tions of the cornea. It seemed to be identical with an organism 

 that zur Nedden had found previously in a case of corneal ulcera- 

 tion in the clinic at Bonn. 



Morphology. It is a tiny bacillus, less than i M in length, slightly 

 curved, generally single, but sometimes in pairs and short chains. 

 It is not motile, has no flagella, forms no spores. 



Staining. It stains ordinarily, but not by Gram's method. 



Cultivation. It is easily cultivated upon the ordinary laboratory 

 media, the cultures being without characteristic peculiarities. 

 Gelatin is not liquefied. Milk is coagulated. Acid but no gas is 

 formed in glucose media. A thick yellowish growth appears upon 

 potato. No indol is formed. 



Pathogenesis. Corneal ulcers were formed in a guinea-pig 

 after artificial implantation in the corneal tissue. 



*" Inaugural Dissertation," Bonn, 1902. 



