710 



Glanders 



Gelatin is not liquefied. The growth upon the surface is grayish 

 white and slimy, never abundant. 



Agar-agar. 'Upon agar-agar and glycerin agar-agar the growth 

 occurs as a moist shining viscid layer. 



Blood-serum. Upon blood-serum the growth is rather character- 

 istic, the colonies along the line of inoculation appearing as cir- 

 cumscribed, clear, transparent drops, which later become confluent 

 and form a transparent layer unaccompanied by liquefaction. 



Potato. The most characteristic growth is upon potato. It first 

 appears in about forty-eight hours as a transparent, honey-like, 

 yellowish layer, developing only at incubation temperatures, and 

 soon becoming reddish-brown in color. As this brown color of the 

 colony develops, the potato for a considerable distance around it 

 becomes greenish brown. Bacillus pyocyaneus sometimes produces 

 somewhat the same appearance. 



Fig. 287. Culture of glanders upon cooked potato (Loffler). 



Milk. In litmus milk the glanders bacillus produces acid. A 

 firm coagulum forms and subsequently separates from the clear 

 reddish whey. 



Metabolic Products. The organism produces acids and curdling 

 ferments. It forms no indol, no liquefying or proteolytic ferments. 

 There is no exotoxin. All the poisonous substances seem to be 

 endotoxins. 



Mallein. Babes,* Bonome,f Pearson, { and others have prepared 

 a substance, mallein, from cultures of the glanders bacillus, and have 

 employed it for diagnostic purposes. It seems to be useful in veteri- 



* "Archiv de Med. exp. et d'Anat. patholog.," 1892, No. 4. 



"Deutsche med. Woch.," 1894, Nos. 36 and 38, pp. 703, 725, and 744. 

 j "Jour, of Comp. Med. and Vet. Archiv," Phila., 1891, xn, pp. 411-415. 



