746 Mycetoma, or Madura-foot 



Cultural Characteristics. Enucleated granules carefully washed 

 in sterile bouillon and then planted upon agar-agar afforded cultures 

 of the mold in 25 out of 65 attempts. 



The growth began in five or six days, appearing on solid media 

 as a tuft of delicate whitish filaments, springing from the black grain, 

 and in a few days covering the entire surface of the medium with a 

 whitish or pale brown felt- work. Upon potato this felt- work sup- 

 ports drops of brownish fluid. The long branched hyphae thus 

 formed were from 3 to 8 M in diameter, with transverse septa in the 

 younger ones. The older hyphae were swollen at the ends. No buds 

 were observed. No fruit organs were detected. In fluid media the 

 filaments radiated from the central grain with the formation of a 

 kind of puff-ball. Eventually the whole medium becomes filled with 

 mycelia and a definite surface growth forms. 



The general characteristics of the fungus are well shown in the 

 accompanying illustrations from Wright's paper. 



