CONTENTS. 



INTRODUCTION 1 1 



Historical Sketch 18 



PART I. 



BACTERIOLOGICAL TECHNIQUE. 

 CHAPTER I. 



PAGE. 



Examination of Bacteria with the Microscope, including Methods of 

 Staining 29 



CHAPTER II. 

 Sterilization ur 



CHAPTER III. 

 Culture-media 7 1 



CHAPTER IV. 



The Cultivation of Bacteria. Tube-cultures ; the Incubator ; Anaer- 

 obic Methods 84 



CHAPTER V. 



The Cultivation of Bacteria (Continued). Isolation of Bacteria; 



Plate-cultures 0.6 



CHAPTER VI. 

 Inoculation of Animals. Autopsies ; Collodion Sacs 103 



CHAPTER VII. 

 Collection of Material 108 



CHAPTER VIII. 



Systematic Study of Species of Bacteria. Suggestions for Class- 

 work; Rules 112 



ix 



