3 MANUAL OF BACTERIOLOGY. 



sufficient, however, when applied in this manner, to kill all 

 kinds of bacteria, especially those containing spores. After 

 it has been passed through the flame three times the 

 preparation may be stained with one of the aniline dyes, 

 and after washing in water and drying may be mounted, 



FIG. 8. 



_D 



Stewart forceps for cover-glasses. 



face down, in Canada balsam upon a glass slide. It makes 

 a suitable object to be examined with the oil-immersion 

 objective. The slide is a thin slip of glass, 3 inches by i 

 inch, with ground edges. 



The smear preparation may equally well be made directly 

 upon the glass slide. The fixation in the flame must then 

 occupy a longer time than with the small and thin cover- 



FIG. 9. 



Kirkbride forceps for holding slides. 



glass. Such preparations have the advantage that several 

 may be made upon one slide, and that after staining them 

 they" may be examined in cedar-oil, with the oil-immersion 

 lens, without the use of the cover-glass and Canada balsam. 

 The forceps of Kirkbride will be found convenient when 

 staining on the slide. Experiments performed in the 



