50 MANUAL OF BACTERIOLOGY. 



(e) Dehydrate in absolute alcohol. 

 (/) Clear in xylol. 

 (g) Mount in balsam. 



GLYCERIN-ALBUMEN MIXTURE (MAYER). 



Equal parts of white of egg and glycerin are thoroughly mixed, and 

 then filtered. Add a little gum-camphor to preserve. 



Section Cutting. Cutting is best done with an instru- 

 ment called a microtome. The tissues may be imbedded in 

 collodion or paraffin ; or when they have been hardened with 

 formaldehyde they may be cut after freezing. Bacteria 



Fie. 10. 



Schanze microtome. 



stain admirably in fro/en sections. Kor routine work col- 

 lodion imbedding will be found as convenient a process as 

 any. Paraffin imbedding gives the thinnest sections. 



A microtome consists of a heavy, sliding knife-carrier, 

 which moves with great precision on a level, and of a de- 



