MANUAL OF BACTERIOLOGY. 



FIG. 26. 



of a 6 per cent, solution of sodium or potassium hydroxide, 

 for each 100 c.c. of air contained in the jar. Add one gram 

 of pyrogallic acid for each 10 c.c. of solution. The culture- 

 tube is placed inside of the 

 larger bottle or tube, supported 

 above the bottom, and the stop- 

 per, smeared with paraffin, is 

 inserted. The mixture of pyro- 

 gallic acid and potassium hy- 

 droxide possesses the property 

 of absorbing oxygen. 



ITright's Modification of 

 BiicJiucr's method: The tube of 

 culture-medium is to be plug- 

 ged with absorbent cotton, us- 

 ing a plug of large size. The 

 culture-medium is inoculated 

 in the usual way. The plug 

 is cut off close to the neck of 

 the tube, and is then pushed 

 into the tube about I centi- 

 meter. Now allow a watery 

 solution of pyrogallic acid to 

 run into the plug, and then a 

 watery solution of sodium or 

 potassium hydroxide. Close 

 quickly and tightly with a rub- 

 ber stopper. Wright recommends that the first solution 

 be freshly made and consist of about equal volumes of 

 pyrogallic acid and water, and that the second solution con- 

 tain I part of sodium hydroxide and j parts of water. With 

 6 inch test-tubes, : | inch diameter, the amounts advised are 

 - .1 c.C. solution of pyrogallic acid, I c.c. solution of sodium 

 hydroxide. 



Arrangement of Tubes for Cul- 

 tivation of Anaerobes by 

 Buchner's Method. 



