IO4 MANUAL OF BACTERIOLOGY. 



wound may be covered with collodion. The peritoneal 

 p> 1G - cavity of the mouse may be inoculated with a fluid 

 culture introduced with a sterile hypodermic 

 syringe. 



Guinea-pigs and rabbits, after inoculation, are to 

 be kept in cages of galvanized iron and wire- 

 netting. The bottom may conveniently be made in 

 the form of a movable pan which permits of the 

 disinfection of the excreta. Rabbits and guinea- 

 pigs may be fed with oats, carrots, cabbage, grass 

 and the like. Guinea-pigs and rabbits may be held 

 by an assistant or tied by the legs upon a board. 

 The hair over a small portion of the abdomen is cut 

 away and a short incision is made through the skin : 

 a pocket is produced \vith a stiff wire, and the ma- 

 terial inserted with a sterile platinum wire. The 

 wound may be covered with collodion. Sutures 

 may be used if the wound is large. Solid sub- 

 stances may conveniently be introduced by placing 

 them in a sterile glass cannula, which is pushed to 

 the proper situation through a small incision. The 

 substance in the cannula is forced out of it with a 

 stiff sterile platinum wire. (Fig. 35.) The peri- 

 toneal cavity may be inoculated with a previously 

 sterilized hypodermic syringe, or an incision may 

 be made which reaches to the peritoneal cavity, 

 into which the desired substance may be introduced 

 with a sterile platinum wire, the incision being 

 closed with sutures. 



Intravenous inoculation is most commonly prac- 

 ticed upon rabbits. A small vein which is near the 

 posterior margin of the ear of the rabbit is easily 

 reached from the dorsal surface; the hypodermic 

 needle is introduced directly into this vein. In making a 



