112 MANUAL OF BACTERIOLOGY. 



CHAPTER VIII. 



SYSTEMATIC STUDY OF SPECIES OF BACTERIA. 1 



IN order to conduct the study of any species of bacteria 

 it is necessary to have the organism isolated in a pure cul- 

 ture by the plate-method, or by some other method already 

 described. Having thus obtained the organism in pure 

 culture, it is to be examined with reference to its behavior 

 in certain particulars. It is well for the beginner to study 

 a few known species of saprophytes obtained from some 

 reliable laboratory in pure culture. The points which are 

 to be considered can be illustrated best by presenting them 

 in tabular form, filling out the items of the table for a 

 given species of bacteria. 



1. Name. 



2. Habitat or source. 



3. Morphology; grouping, as in chains or in zoogloe^e. 



4. Size. 



5. Staining proporties. Behavior by Gram's Method. 



6. Capsule, present or otherwise. 



7. Spore formation. 



8. Motility, flagella. 



Growth on culture-media. 



9. Relation of growth to temperature. 



10. Gelatin; observe whether the gelatin is liquefied or 

 not. Colonies in gelatin plates, study under low 

 power of microscope. 



1 For the identification of unknown species consult "A Manual of 

 Determinative Bacteriology," by Frederick D. Chester. 



